Colorimetric assay kit

Manufactured by Elabscience

Sourced in United States, China

The Colorimetric assay kit is a laboratory equipment designed to perform quantitative analysis of various analytes in a sample. It utilizes the principles of colorimetry, where the concentration of a substance is determined by the intensity of the color produced in a chemical reaction. The kit includes all the necessary reagents and consumables required to conduct the assay.

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Lab products found in correlation

Mda colorimetric assay kit, by Elabscience (2 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Colorimetric assay kit, by Abcam (1 mentions) Ph test strips, by Macherey-Nagel (1 mentions) Osmomat 3000, by Gonotec (1 mentions) Bs 120 chemistry analyzer, by Mindray (1 mentions) Elisa assay kit, by Elabscience (1 mentions) Spss version 23, by IBM (1 mentions) Sandwich elisa, by BioAssay Systems (1 mentions) Cobas c311, by Roche (1 mentions) Peg cat, by Merck Group (1 mentions) Dtx880 multimode detector microplate reader, by Beckman Coulter (1 mentions) Amplex red hydrogen peroxide peroxidase assay kit, by Thermo Fisher Scientific (1 mentions) E bc k025 s, by Elabscience (1 mentions) Rat elisa kit, by MyBioSource (1 mentions) Rat elisa kit, by Cusabio (1 mentions) Cobas c702, by Roche (1 mentions) Triglyceride determination kit, by Merck Group (1 mentions) Xanthine oxidase activity assay kit, by Merck Group (1 mentions) Interleukin 6 (il 6), by Diaclone (1 mentions)

Close spelling variants of this product

Cell Ferrous Iron Colorimetric Assay Kit (9 citations) MDA) Colorimetric Assay Kit (8 citations) L-Lactic Acid Colorimetric Assay Kit (6 citations) Cell Total Iron Colorimetric Assay Kit (5 citations) Iron Colorimetric Assay Kit (5 citations) Triglyceride colorimetric assay kit (5 citations) Reduced Glutathione (GSH) Colorimetric Assay Kit (4 citations) Total Cholesterol Colorimetric Assay Kit (3 citations) Glucose colorimetric assay kit (3 citations) NEFA Colorimetric Assay Kit (3 citations)

28 protocols using colorimetric assay kit

Biomarkers for Renal Function Assessment

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Renal functional assessment biomarkers such as serum creatinine (µmol/L), albumin (g/dL), urea (mg/dL) and uric acid (µmol/L) were evaluated using commercially available colorimetric assay kits (Elabscience Biotechnology Co. Ltd., Wuhan, China). BUN is calculated by using the following formulae:

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$${\rm{Urea }}\left[{{\rm{mmol/L}}} \right]{\rm{ = BUN }}\left[{{\rm{mg/dL}}} \right]{\rm{ x10 }}\left[{{\rm{dL/L}}} \right]{\rm{/ 14x2 }}\break\left[{{\rm{mg\ N/mmol\ urea}}} \right]$$
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Further, Urinary protein concentrations (mg/day) were evaluated by bicinchoninic acid (BCA) assay method using BSA as standard (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Guo J., Li J., Wei H, & Liang Z. (2021). Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways. Drug Design, Development and Therapy, 15, 4339-4358.

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Colorimetric Assay for Plasma MDA

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Detection of the malondialdehyde (MDA) content can reflect the level of lipid peroxidation in cells and indirectly reflect the level of cellular damage. Colorimetric assay kits were used according to the manufacturer’s instructions (Elabscience, Houston, Texas, USA). Fresh blood samples were stored in a tube with anticoagulant and centrifuged at 14,000 rpm for 10 min at 4 °C. Clear plasma without any white blood cells and platelets were separated. The plasma samples were stored at −80 °C for up to a month. All of the samples were measured at an OD of 532 nm, with 1 cm optical path cuvette, and the values were given as nmol/mL.
The amount of MDA was calculated using the given formula:

Sherwani S., Raafat M., Rajendrasozhan S., Khan M., Saleem M., Husain Q., Khan S., Alam N, & Khan M.W. (2022). Increased Levels of Autoantibodies against ROS-Modified Proteins in Depressed Individuals with Decrease in Antibodies against SARS-CoV-2 Antigen (S1-RBD). Current Issues in Molecular Biology, 44(11), 5260-5276.

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Comprehensive Renal Biomarker Profiling

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The pH of urine was measured using pH test strips (Macherey‐Nagel GmbH & Co. KG, Düren, Germany). Urine osmolality was measured using a freezing point osmometer (Osmomat 3000, Gonotec GmbH, Berlin, Germany). All of the tests in plasma, urine and renal homogenates were done using standard ELISA or colorimetric assay kits. Biomarkers of renal function (urea and creatinine) and magnesium in plasma and uric acid and phosphorus in both urine and plasma were all measured using an automated machine (Mindray BS‐120 Chemistry Analyzer, Shenzhen Mindray Bio‐Medical Electronics Co., Shenzhen, China). Cysteine, sodium and potassium were measured using colorimetric assay kits from Elabscience (Houston, TX, USA). Calcium, oxalate, citrate and phosphate were estimated using colorimetric assay kits from BioVision (Milpitas, CA, USA).¹⁵

Al‐Marhoon M.S., Al‐Harrasi A., Siddiqui K., Ashique M., Ali H, & Ali B.H. (2023). Effects of frankincense on experimentally induced renal stones in rats. BJUI Compass, 4(4), 437-445.

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Enzymatic Activity and Oxidative Stress

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We measured the enzymatic activity of lactate dehydrogenase (LDH; Solarbio, China) and the concentrations of malondialdehyde (MDA; Beyotime, China) and H₂O₂ using appropriate colorimetric assay kits (Elabscience, China).

Li D., Zhang D., Tang B., Zhou Y., Guo W., Kang Q., Wang Z., Shen L., Wei G, & He D. (2019). Exosomes from Human Umbilical Cord Mesenchymal Stem Cells Reduce Damage from Oxidative Stress and the Epithelial-Mesenchymal Transition in Renal Epithelial Cells Exposed to Oxalate and Calcium Oxalate Monohydrate. Stem Cells International, 2019, 6935806.

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Antioxidant Responses in Plant Roots

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Hydrogen peroxide (H₂O₂) levels, L-ascorbate peroxidase (APX) activity, and peroxidase (POD) activity in roots, and glutathione (GSH) levels in roots and shoots were measured using colorimetric assay kits (Elabscience, China), according to the manufacturer’s instructions. H₂O₂ levels, APX activity, POD activity, and GSH levels were expressed as mmoL/g FW, U/g FW, U/mg protein, and μmoL/mg protein, respectively.

Yue Z., Chen Y., Wang Y., Zheng L., Zhang Q., Liu Y., Hu C., Chen C., Ma K, & Sun Z. (2022). Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism. Frontiers in Plant Science, 13, 941388.

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Cardiac Tissue Homogenization and Biomarker Analysis

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The hearts were taken out, cleaned, and weighed before being sliced into apical and basal sections. Clots were removed by rinsing the basal side of the heart with ice-cold saline and then stored in a deep freeze (-80 C). Each part was weighed after melting and homogenized using an ultrasonic liquid processor with high intensity in 1:10 (w/v) 0.1-M phosphate-buffered saline (pH-7.4) containing 1% triton x-100 and protease-inhibitors cocktails [18 (link)]. The homogenate was centrifuged at 5,000 rpm at 4℃ for 10 minutes. The supernatants were utilized to evaluate malondialdehyde (MDA) level, caspase-3, and Bcl-2 using available ELISA kits and total antioxidant capacity (TAC) using colorimetric assay kits as directed by the manufacturer guidelines (Elabscience, U.S.A.).

Alyasiry E., Janabi A, & Hadi N. (2022). Dipyridamole ameliorates doxorubicin-induced cardiotoxicity. Journal of Medicine and Life, 15(9), 1184-1190.

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Biochemical Markers in Biomedical Research

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Serum creatinine, blood urea nitrogen (BUN), calcium, and phosphate were measured by colorimetric assay kits (Elabscience). Serum PTH was tested by an enzyme-linked immunosorbent assay (ELISA) kit (Elabscience).

Fu R., Meng K., Zhang R., Du X, & Jiao J. (2023). Bone marrow-derived exosomes promote inflammation and osteoclast differentiation in high-turnover renal osteodystrophy. Renal Failure, 45(2), 2264396.

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Serum Biomarker Analysis in Mice

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At the end of the experiment, mouse blood was collected by cardiac puncture, and the serum was separated by centrifugation. Blood urea nitrogen (BUN) and creatinine were measured using colorimetric assay kits (Elabscience, Wuhan, China) according to the manufacturer's protocol.

Ran F., Yang Y., Yang L., Chen S., He P., Liu Q., Zou Q., Wang D., Hou J, & Wang P. (2022). Capsaicin Prevents Contrast-Associated Acute Kidney Injury through Activation of Nrf2 in Mice. Oxidative Medicine and Cellular Longevity, 2022, 1763922.

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Pyruvate Measurement in Cell Lysates

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The levels of Pyruvate were measured by using colorimetric assay kits, according to the manufacturer's instructions (ElabScience, USA). The cells lysates and conditioned media of treated cells with the least synergistic doses and untreated cells were collected after 48 h. The OD values were measured of each sample at 505 nm in spectrophotometer.

Al-Shammari A.M., Abdullah A.H., Allami Z.M, & Yaseen N.Y. (2019). 2-Deoxyglucose and Newcastle Disease Virus Synergize to Kill Breast Cancer Cells by Inhibition of Glycolysis Pathway Through Glyceraldehyde3-Phosphate Downregulation. Frontiers in Molecular Biosciences, 6, 90.

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Oxidative Stress Biomarkers Quantification

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The levels of oxidative stress biomarkers, including Superoxide dismutase (SOD), Malondialdehyde (MDA), 8-oxo-deoxyguanosine (8-OHdG) and catalase (CAT) in serum of patients or animals were detected using an enzyme-linked immunosorbent assay (ELISA) assay kits and Colorimetric Assay Kits (Elabscience Wuhan, China).

Lv Y., Zhao X., Zhang R., He Z., Xu Y., Tu L., Jiang L., Xu S, & Zhang Q. (2023). Oxidative stress mediates hippocampal neuronal apoptosis through ROS/JNK/P53 pathway in rats with PTSD triggered by high-voltage electrical burn. Folia morphologica.

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