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Cd4 alexa 594

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The CD4 Alexa 594 is a fluorescently labeled antibody that specifically binds to the CD4 receptor expressed on the surface of T helper cells. It is a tool used for the identification and analysis of CD4+ T cells in flow cytometry applications.

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Lab products found in correlation

Cd3 alexa 488, by BioLegend (3 mentions) Cd8 alexa 647, by BioLegend (3 mentions) Hoechst dye, by Merck Group (3 mentions)

3 protocols using cd4 alexa 594

1

Immunophenotyping of Tumor-Infiltrating T Cells

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Resected tumors were fixed for 2 h in 2% paraformaldehyde and incubated in 30% sucrose overnight. Sections were cut (5 µm) and stained with combined primary antibodies CD3 Alexa 488 (100,212, Biolegend), CD4 Alexa 594 (100,446, Biolegend), and CD8 Alexa 647 (100,727, Biolegend) and nuclei were labeled with Hoechst dye (bis benzimide, Sigma B-2283; 1 mg/100 ml in dH20). Images were acquired digitally from nine fields under each condition. Density of positive cells was evaluated by automated image analysis using Nikon Elements (Nikon Instruments Inc, Melville, NY). Percentage of CD3+ T cells, CD3+CD4+, and CD3+CD8+ T cells per area was calculated using the number of cells positive for the antibody versus the total number of cells. Student’s t-test was used to analyze statistical significance.
Yang M., Giehl E., Feng C., Feist M., Chen H., Dai E., Liu Z., Ma C., Ravindranathan R., Bartlett D.L., Lu B, & Guo Z.S. (2021). IL-36γ-armed oncolytic virus exerts superior efficacy through induction of potent adaptive antitumor immunity. Cancer Immunology, Immunotherapy, 70(9), 2467-2481.
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2

Quantifying Tumor-Infiltrating T-Cell Subsets

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Resected tumors were fixed for 2 h in 2% Paraformaldehyde and incubated in 30% sucrose overnight. Sections were cut (5 μm) and stained with combined primary antibodies CD3 Alexa 488 (100212, BioLegend), CD4 Alexa 594 (100446, BioLegend) and CD8 Alexa 647 (100727, BioLegend) and nuclei were labeled with Hoechst dye (bis benzimide, Sigma B-2283–1 mg/100 ml in dH20). Images were acquired digitally from 9 fields under each condition. Density of positive cells was evaluated by automated image analysis using Nikon Elements (Nikon Instruments Inc, Melville, NY). Percentage of CD3+ T cells, CD3+CD4+, CD3+CD8+ T cells per area has been calculated by number of cells positive for the antibody versus the total number of cells.
Feist M., Zhu Z., Dai E., Ma C., Liu Z., Giehl E., Ravindranathan R., Kowalsky S.J., Obermajer N., Kammula U.S., Lee A.J., Lotze M.T., Guo Z.S, & Bartlett D.L. (2020). Oncolytic Virus Promotes Tumor-Reactive Infiltrating Lymphocytes for Adoptive Cell Therapy. Cancer gene therapy, 28(1-2), 98-111.
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3

Quantifying Tumor-Infiltrating T-Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Resected tumors were fixed for 2 h in 2% Paraformaldehyde and incubated in 30% sucrose overnight. Sections were cut (5 μm) and stained with combined primary antibodies CD3 Alexa 488 (100212, BioLegend), CD4 Alexa 594 (100446, BioLegend) and CD8 Alexa 647 (100727, BioLegend) and nuclei were labeled with Hoechst dye (bis benzimide, Sigma B-2283–1 mg/100 ml in dH20). Images were acquired digitally from 9 fields under each condition. Density of positive cells was evaluated by automated image analysis using Nikon Elements (Nikon Instruments Inc, Melville, NY). Percentage of CD3+ T cells, CD3+CD4+, CD3+CD8+ T cells per area has been calculated by number of cells positive for the antibody versus the total number of cells.
Feist M., Zhu Z., Dai E., Ma C., Liu Z., Giehl E., Ravindranathan R., Kowalsky S.J., Obermajer N., Kammula U.S., Lee A.J., Lotze M.T., Guo Z.S, & Bartlett D.L. (2020). Oncolytic Virus Promotes Tumor-Reactive Infiltrating Lymphocytes for Adoptive Cell Therapy. Cancer gene therapy, 28(1-2), 98-111.
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