Human Protein Atlas (https://www.proteinatlas.org ) was interrogated for progranulin and Ephrin-A1 expression in bladder and urothelial carcinoma tissues in September 2018, and representetive images were acquired and compared to highlight different expression levels among the analyzed tissues. EphA2 expression was analyzed by immunohistochemistry as described [21 (link),22 (link),104 (link)] on a Biomax human bladder cancer tissue microarray (BL208) at the Translational Core Facility of the Sidney Kimmel Cancer Center. The antibody used was an anti-EphA2 rabbit polyclonal antibody (Santa Cruz Biotechnology (C-20): sc-924) at a dilution of 1:100. Detailed specifications of the bladder tissue array can be found at: http://www.biomax.us/tissue-arrays/Bladder/BL208 . The array contains 43 cases of urothelial carcinoma, 7 adenocarcinoma, 6 carcinoma in situ, 4 metastatic carcinoma and 9 bladder tissue, triplicate cores per case. Quantification of EphA2 staining in the microarray was done utilizing ImageJ. The threshold of staining of each image acquired was adjusted in order to show only the specific staining. The representative areas of staining were then quantified and a representative plot was created in SigmaPlot.
Human bladder cancer tissue microarray
Manufactured by Tissue Array
Sourced in United States
The Human Bladder Cancer Tissue Microarray is a collection of small tissue samples from multiple bladder cancer specimens mounted on a single microscope slide. This array allows for the simultaneous analysis and comparison of molecular expressions or alterations across a large number of bladder cancer cases.
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2 protocols using human bladder cancer tissue microarray
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Quantitative Analysis of EphA2 in Bladder Cancer
2
Progranulin Expression in Bladder Cancer
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Progranulin expression was analyzed by immunohistochemistry on a Biomax (Rockville, MD, USA) human bladder cancer tissue microarray (BL208) at the Translational Core Facility of the Sidney Kimmel Cancer Center. The antibody used was an anti-granulin rabbit monoclonal antibody (Abcam, ab108608) at a dilution of 1:400. Detailed specifications of the bladder tissue array can be found at: http://www.biomax.us/tissue-arrays/Bladder/BL208 .
Archived paraffin-embedded tissues from 4 non-invasive carcinoma in-situ (Tis), 4 non-invasive low grade papillary carcinomas (Ta), 4 non-invasive high grade papillary carcinomas (Ta) and control benign and invasive high grade carcinoma tissues (n = 4) were stained for progranulin expression as described above. All cases in the tissue microarray and from archived samples were analyzed for progranulin expression and quantified. Briefly, the threshold of each image was adjusted in order to show only progranulin-specific staining. The background was removed and the data were quantified and plotted using SigmaPlot software. Student's t-tests were run to determine statistical significance of each class of cancers compared to normal bladder.
Archived paraffin-embedded tissues from 4 non-invasive carcinoma in-situ (Tis), 4 non-invasive low grade papillary carcinomas (Ta), 4 non-invasive high grade papillary carcinomas (Ta) and control benign and invasive high grade carcinoma tissues (n = 4) were stained for progranulin expression as described above. All cases in the tissue microarray and from archived samples were analyzed for progranulin expression and quantified. Briefly, the threshold of each image was adjusted in order to show only progranulin-specific staining. The background was removed and the data were quantified and plotted using SigmaPlot software. Student's t-tests were run to determine statistical significance of each class of cancers compared to normal bladder.
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