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2 protocols using stat6tyr641

1

STAT Signaling Pathway Antibodies

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Phospho-specific antibodies to STAT1Tyr701, STAT3Tyr705, STAT5Tyr694 and STAT6Tyr641 were from Cell Signaling Technologies (Beverly, MA, USA). STAT1, STAT3, STAT5, STAT6, JAK1, JAK2, JAK3, PTPN6, Bcl-2, c-Myc, Mcl-1 and survivin antibodies were also from Cell Signaling Technologies. Actin antibody was purchased from Santa Cruz (Santa Cruz, CA, USA). Recombinant human IL-2, IL-6 and IL-10 were from Peprotech (Rocky Hill, NJ, USA). IFN-α was purchased from Sigma Aldrich (St. Louis, MO, USA).
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2

Cytotoxicity Evaluation of 3-DSC

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3-DSC (purity >98%) was purchased from AK Scientific, Inc (Union City, CA, USA). CellTiter-Glo®Luminescent Cell Viability Assay kit was purchased from Promega Corporation (Madison, WI, USA). DMEM and fetal bovine serum (FBS) were procured from Invitrogen (Carlsbad, CA, USA). Interleukin (IL)-6 and IL-4 were purchased from R&D systems (Minneapolis, MN, USA). β-actin antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). Polyclonal antibodies against total β-catenin, phospho-specific β-catenin (Thr41/Ser45), total STAT3, STAT6, phospho-specific STAT3 (Tyr705) and STAT6 (Tyr641) were purchased from Cell Signaling Technology (Beverly, MA, USA). All other chemicals used in our experiments were molecular biology grade.
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