For the secondary and tertiary TS formation assays, primary TS were mechanically dissociated after which 200.000 cells were cultured in ultralow attachment 100 mm plates (Falcon) in the presence of JQ1 (200 nM) for 3 days. Next, the number of secondary TS per plate were determined. Secondary TS were again dissociated and left in the presence of the drug until day 6, at which the number of tertiary TS per plate was determined. TS pictures were taken at the two time points using an inverted microscope (Nikon).
Ultralow attachment 100 mm plates
The Ultralow attachment 100 mm plates are a type of laboratory equipment designed for cell culture applications. The plates have a specialized surface treatment that inhibits cell attachment, promoting the formation of cell aggregates or spheroids. This feature is useful for various cell-based assays and research requiring the maintenance of three-dimensional cell structures.
Lab products found in correlation
2 protocols using ultralow attachment 100 mm plates
Limiting Dilution Assay for Breast Cancer Stem Cells
For the secondary and tertiary TS formation assays, primary TS were mechanically dissociated after which 200.000 cells were cultured in ultralow attachment 100 mm plates (Falcon) in the presence of JQ1 (200 nM) for 3 days. Next, the number of secondary TS per plate were determined. Secondary TS were again dissociated and left in the presence of the drug until day 6, at which the number of tertiary TS per plate was determined. TS pictures were taken at the two time points using an inverted microscope (Nikon).
Synergistic Effects of JQ1 and Volasertib on Tumorsphere Formation
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