Cell proliferation reagent

Zein powder (MW 20 kDa),
ethanol (≥99.7%), l-ascorbic acid (Vit C), phosphate-buffered
saline (PBS) 1×, sodium acetate, calcium chloride (CaCl₂), apramycin sulfate salt, trifluoroacetic acid (TFA), and ethanol/hexamethyldisilizane
(36%) were purchased from Sigma-Aldrich and used as received. Low
methoxy pectin powder was purchased from Silva Team. Lecithin (90%)
soybean was purchased from Alfa Aesar. The cell proliferation reagent
[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium] (MTS) and the CellTiter-Glo Luminescent viability
assay kit were obtained from Promega. HDFa, fibroblast basal medium
supplemented with Supplement Pack Fibroblast Growth Medium 2, 0.25%
trypsin–EDTA (1×), 2-(4-aminiodinophenyl)-6-idolecarbamide
dihydrochloride (DAPI), and Alexa Fluor 488 Phalloidin were purchased
from Thermo Fisher Scientific. HaCaT cells were purchased from the
Cell Line Service (Heidelberg, Germany).

3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy- phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays were performed to determine relative cell proliferation. Cells were plated in a 96-well plate and transfected with negative control siRNA or DRG1 siRNA. At 0 h, 24 h, 48 h, and 72 h after transfection, the cells were incubated in Cell Proliferation Reagent (Promega) according to manufacturer's instructions.