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5 protocols using sodium tetraborate decahydrate

1

Synthesis of Ionic Polymer Hydrogel

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IA (itaconic acid) and SSS (sodium p-styrene sulfonate) used in the experiments were purchased from Shandong Usolf Chemical Technology Co., Ltd. in China, AM was supplied by Shanghai Aladdin Biochemical Technology Co., Ltd. in China, and ammonium persulfate ((NH4)2S2O8) was purchased from Shanghai Macklin Biotechnology Co. Other anhydrous calcium chlorides, sodium bicarbonate, potassium hydroxide, potassium chloride, sodium tetraborate decahydrate, disodium EDTA and calcium carboxylate were purchased from Sinopharm (Shanghai, China) Chemical Reagent Co., IA, and SSS were of technical grade, all other chemicals were of analytical reagent grade, all solutions were prepared using deionized water.
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2

Preparation of Chitosan-Sodium Alginate Fabric

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CS (viscosity 50–800 m Pa s, degree
of deacetylation 80–95%) and SA (Mw: 50,075 kDa) were purchased from Sinopharm Reagent Co., Ltd. (Shanghai,
China). Sodium tetra-borate decahydrate (Na2B4O7·10H2O), nickel acetate tetrahydrate
(C4H6O4Ni·4H2O),
and ferric chloride hexahydrate (FeCl3·6H2O) were also acquired from Sinopharm Chemical Reagent Co., Ltd. Sodium
hydroxide and concentrated hydrochloric acid (HCl, 36.5–38%)
were received from Changzheng Chemical Reagent Corporation. 100% PA66
woven fabrics with a yarn count 135 denier, Ends per inch (EPI) 85,
Picks per inch (PPI) 81, and density of 100 g/m2 were obtained
from Yangzhou City Jiaxing Weaving Industry Co. Ltd. (China). The
scoured and air-dried fabric samples were used in the experimental
process.
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3

Synthesis and Characterization of Hyaluronic Acid

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The high-molecular-weight HA sample (2000 kDa) and low-molecular-weight sample (200 kDa) were purchased from Bloomage Freda Biopharm Co. Ltd. (Shandong, China); 1,4-butanediol diglycidyl ether (BDDE), (methyl sulfoxide)-d6 and d-glucuronic acid were purchased from Meryer (Shanghai) Chemical Technology Co., Ltd. (Shanghai, China); NaOH, KOH, NaH2PO4·2H2O, Na2HPO4·12H2O, sodium tetraborate decahydrate and nicotinamide were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China); hyaluronidase was purchased from Shanghai yuanye Bio-Technology Co. Ltd. (Shanghai, China); H2SO4 was purchased from Shanghai Lingfeng Chemical Reagent Co. Ltd (Shanghai, China); carbazole and formic acid were purchased from Shanghai Aladdin Bio-Chem Technology Co. Ltd (Shanghai, China); acetophenone was purchased from Energy Chemical (Shanghai, China). All the chemicals were of analytical grade.
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4

Analyzing Flowering Cabbage Chemical Properties

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Fresh Chinese flowering cabbage was purchased from a local supermarket (Wuxi, China). Indicators (bromocresol green, methyl red sodium salt, bromophenol blue, chlorophenol red, bromothymol blue sodium, and bromocresol purple), sodium hydroxide (NaOH), ethanol, phenolphthalein, sodium tetraborate decahydrate, potassium hexacyanoferrate (II) trihydrate, zinc acetate dihydrate, silver nitrate solution, and qualitative filter paper were bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). N–(1–Naphthyl) ethylenediamine dihydrochloride, t-butylammonium hydroxide (TBAH), and p-toluene sulfonic acid (TsOH) were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). 5,5–dithiobis (2–nitrobenzoic acid; DTNB) was bought from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China), and polyethylene glycol (PEG–400) was bought from Beijing InnoChem Science and Technology Co., Ltd. (Beijing, China). Man, Rogosa, and Sharpe (MRS) broth was purchased from Haibo Biotechnology Co., Ltd. (Qingdao, Shandong, China). Polyvinylidene fluoride (PVDF) membranes were purchased from Biosharp Biotechnology Co., Ltd. (Shanghai, China). All chemical reagents were of analytical grade and used as received.
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5

Synthesis of Graphene-Based Composites

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Sodium sulfate decahydrate (Na2SO4·10H2O, analytical reagent (AR), purity >99%), potassium chloride (KCl, AR, purity >99%), sodium tetraborate decahydrate (Na2B4O7·10H2O, AR, purity >99%), and polyacrylamide ((C3H5NO)n, solid content >90%, molecular weight ≥10 million) were offered by the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Expanded graphite (expanded ratio: 234 mL/g, carbon content: 99%) was purchased from the Qingdao Taixing Graphite Co., Ltd. (Qingdao, China).
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