Cells harvested after overnight incubation were stained following standard protocols. Experiments used antibodies specific for human FcR block (BioLegend #422302), CD11c (Bu15, BioLegend #337214), CD80 (2D10, BioLegend #305220), CD86 (Bu63, BioLegend #374210) HLA-DR (L243, BioLegend #307629), HLA-A,B,C (W6/32, BioLegend #311410), CD14 (M5E2, BioLegend #301828), CD63 (H5C6, BioLegend #353007), CD61 (VI-PL2, BioLegend #336416), CD41/CD61 (PAC-1, BioLegend 362804), CD62p (AK4, BioLegend #304906), mTCRb (H57-597, BioLegend #109206), CD4 (OKT4, BioLegend #317428), CD8 (SK1, BioLegend #344704), and human CD3-APC (Clone OKT3, BioLegend). Color-matched isotype control antibodies were obtained from the same vendors. Flow cytometry was performed with a Stratedigm flow cytometer with electronic gates set on live cells by a combination of forward and side light scatter and 7-AAD (BioLegend), EMA (Invitrogen), Zombie Aqua (BioLegend), or Zombie NIR (BioLegend) exclusion. A minimum of 3 × 104 events were collected per sample, and data were analyzed with FlowJo software (FlowJo LLC).
Cd86 bu63
Manufactured by BioLegend
CD86 (Bu63) is a cell surface protein that functions as a costimulatory molecule, playing a role in T-cell activation. It is expressed on various antigen-presenting cells, including dendritic cells, macrophages, and B cells.
Automatically generated - may contain errors
Lab products found in correlation
Zombie aqua, by BioLegend (1 mentions)
Zombie nir, by BioLegend (1 mentions)
7 aad, by BioLegend (1 mentions)
Cd14 m5e2, by BioLegend (1 mentions)
Cd80 2d10, by BioLegend (1 mentions)
H57 597, by BioLegend (1 mentions)
W6 32, by BioLegend (1 mentions)
Cd63 h5c6, by BioLegend (1 mentions)
F4 80 bm8, by BioLegend (1 mentions)
Ly6g 1a8, by BD (1 mentions)
Cd38 90, by BioLegend (1 mentions)
Cd15 hi98, by BD (1 mentions)
Gapdh d16h11, by Cell Signaling Technology (1 mentions)
2 protocols using cd86 bu63
1
Comprehensive Immune Cell Profiling
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies and their corresponding clones used for flow cytometry (FC): anti-human CD11b (M1/70), CD33 (WM53), HLA-DR (G46-6), CD14 (M5E2), and CD15 (HI98) were obtained from BD Biosciences (Woburn, MA). Zombie NIR was used as a cell viability dye and CD86 (BU63) was obtained from (BioLegend, San Diego, CA). FC antibodies used to detect mouse MDSCs and macrophages were Gr1 (Ly6G/Ly6C, RB6-8C5), CD11b (M1/170), Ly6C (AL-21), and Ly6G (1A8) obtained from BD Biosciences, F/480 (BM8), CD38 (90) obtained from BioLegend, and EGR2 (ERONGR2) procured from Thermo Fisher Scientific (Waltham, MA). Antibodies against TLR7 (D7), TLR9 (D2C9), IL6 (D3K2N), phospho-NFκB p65Ser536 (93H1), total NFκB (C22B4), phospho-STAT3Tyr705 (D3A7) and total STAT3 (D3Z26) were obtained from Cell Signaling Technology (Danvers, MA). GAPDH (D16H11) was used for loading control and was obtained from Cell Signaling Technology.
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