Archived FFPE blocks were retrieved from the tissue bank of Picardie of the pathology department. Three μm thick FFPE tissue sections were cut by using a microtome (Thermo Fisher Scientific HM 355S automatic rotary microtome, MMFRANCE®) at room temperature. For each case, stained and nonstained slides were prepared (Matsunami TOMO® hydrophilic adhesion slides, VWR-France). For standard hematoxylin phloxine saffron (HPS) staining, we used the Tissue Tek Film™ machine (Sakura®, France). All the slides were stored at room temperature. The first 3 μm section was stained and the consecutive section was prepared for the SpiderMass analysis. The FFPE tissues for the SpiderMass analysis were manually sprayed with a glycerol/isopropyl alcohol (IPA) (8:2, v/v) solution in two successive passes by using a manual sprayer (Agilent). The syringe pump (74900 series Cole Parmer Instrument Company) was set to a 300-μl/min flow rate. The two successive passes were equal to 5 μl deposited on 1 cm2 as previously described [30 (link)].
Manual sprayer
Manufactured by Agilent Technologies
The Manual Sprayer is a portable, hand-operated device used for applying liquids, such as solutions or dispersions, in a fine mist or spray. It is designed for controlled and targeted application of various substances, including cleaning agents, pesticides, or other liquid formulations. The device consists of a container to hold the liquid and a manual pump or trigger mechanism that atomizes the liquid and propels it through a nozzle, producing a spray.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using manual sprayer
1
FFPE Tissue Preparation for SpiderMass Analysis
2
Tissue Preparation for Mass Spectrometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues were sectioned at 7 µm thickness and deposited onto a polylysine glass slide. Then, the FFPE tissue sections were submitted to dewaxing two times in xylene for 5 min and were manually sprayed with a glycerol/isopropyl alcohol (IPA) (8:2, v/v) solution in 2 successive passes using a manual sprayer (Agilent). The syringe pump (74 900 series Cole Parmer Instrument Company) was set to a 700 µL/min flow rate. The 2 successive passes were equal to 5 µL deposited on 1 cm2 and took ∼10 s. The samples were analyzed within 10 min after the glycerol deposition52.
3
FFPE and Frozen Tissue Preparation for SpiderMass
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the FFPE blocks were sectioned into 8 μm tissue sections using a microtome (Leica Biosystems, Wetzlar, Germany) at room temperature. A dewaxing step was performed through two 5 min washes in a xylene solution. Then, each dewaxed tissue section of the retrospective glioblastoma cohort was manually sprayed with a glycerol/isopropyl alcohol (IPA) (8:2, v/v) solution in two successive passes using a manual sprayer (Agilent). The syringe pump (74900 series Cole Parmer Instrument Company) was set to a 500 μL/min flow rate. As for fresh frozen tissues, a Leica CM1510S cryostat (Leica Microsystems, Nanterre, France) was used to cut 20 μm sections. Their analysis by the SpiderMass does not require any sample preparation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!