Alexa fluor 488 conjugated goat anti mouse igg h l

The mouse polyclonal antibody against CP204L and rabbit polyclonal antibody against B646L were prepared in our laboratory. Polyclonal rabbit anti-SNX32 (catalog no. 25763-1-AP), rabbit anti-p62 (catalog no. 18420-1-AP), and rabbit anti-RAB1B (catalog no. 17824-1-AP) were purchased from Proteintech. Rabbit anti-LC3B (catalog no. 3868s) and mouse anti-Myc (catalog no. 2276S) were purchased from Cell Signaling Technology. Monoclonal mouse anti-HA (catalog no. H3663), mouse IgG polyclonal antibody (catalog no. 12-371), rabbit IgG polyclonal antibody (catalog no. 12-370), and mouse anti-Flag (catalog no. F1804) were purchased from Sigma-Aldrich. Mouse anti-β-actin was purchased from Santa Cruz (catalog no. sc-58673). Alexa Fluor 488-conjugated goat anti-mouse IgG (H+L) (catalog no. 4408s) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H+L) (catalog no. 8889s) antibodies were purchased from Cell Signaling Technology; IPKine goat anti-mouse IgG heavy chain secondary antibody, HRP labeling (elimination of light chain interference) (catalog no. A25112) and IPKine goat anti-mouse IgG light chain secondary antibody, HRP labeling (elimination of heavy chain interference) (catalog no. A25012) were purchased from Abbkine. Dimethyl sulfoxide, 3-MA (autophagosome formation inhibitor), MG132 (proteasome inhibitor), and NH₄Cl (lysosome inhibitor) were purchased from Sigma-Aldrich.

To investigate the morphological changes, BM-MSCs (1 × 10⁵ cells/dish) were seeded on Petri dishes and cultured as described above. After 48 h, cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X/0.1% Tween/1 × PBS (30 m). The cells were then blocked with 2% goat serum (Sigma Aldrich, Schnelldorf, Germany) for 1 h and incubated with rabbit vimentin antibody (1:100 dilution; cat. No. D21H3 XP, Cell Signaling Technology, Beverly, MA, USA) for 1 h. After washing, the cells were incubated with the Alexa Fluor 488-conjugated Goat anti-mouse IgG (H + L) (1:500 dilution; Cell Signaling Technology, USA) for 1 h at room temperature. DAPI was used to stain cell nuclei. Fluorescent images were captured on a Ti-E microscope (Nikon instrument, Melville, NY, USA) at 10× magnification.

Rabbit anti-MGF-505-7R and anti-P30 sera were raised against a recombinant ASFV MGF-505-7R and P30 proteins. Polyclonal rabbit anti STAT1 (#14994), phospho-STAT1 (Tyr701) (#9167), JAK1 (#29261), and JAK2 (#3230) were purchased from Cell Signaling Technology. Polyclonal rabbit anti RNF125 (ab211450) and Hes5 (ab194111) were purchased from Abcam. Monoclonal mouse anti HA (H3663), β-actin (A5441), Myc (SAB1305535), and Flag (F3040) were purchased from Sigma-Aldrich. Alexa Fluor-488-conjugated goat anti-mouse IgG (H + L) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) antibodies were purchased from Cell Signaling Technology. 3-MA (189490) and MG132 (M8699) were purchased from Sigma-Aldrich. Porcine IFN-γ ELISA kit was purchased from Solarbio Life Sciences (SEKP-0010).