Rabbit anti cyclin e1

Rabbit anti-ACAT2 (1:1000) (ab131215) and rabbit anti-P21 (1:1000) (ab109520) were purchased from Abcam. Rabbit anti-SETD7 antibody(1:1000) (24840-1-AP), rabbit anti-P16-INK4A(1:1000) (10883-1-AP), rabbit anti-P27 (1:1000)(25614-1-AP), rabbit anti-Cyclin B1 (1:1000)(55004-1-AP), rabbit anti-Cyclin E1(1:1000)(11554-1-AP), mouse anti-Cyclin D1(1:1000)(60186-1-Ig), rabbit anti-MCM2(1:1000)(10513-1-AP), rabbit anti-Cortactin (1:400)(11381-1-AP) and rabbit anti-SMA (1:1000)(14395-1-AP), mouse anti-Vimentin(1:4000)(60330-1-Ig) were purchased from Proteintech. Rabbit anti-Snail2 (1:1000) (121235) was purchased from Brickell Biotech, Inc. Antibodies against YAP1(1:1000) (A1002), TAZ (1:1000) (A23034), TEAD1(1:1000) (A5218) were purchased from AB clonal. Rabbit anti-vinculin (1:1000) (E1E9V), rabbit anti-flag antibody (1:1000) (2272S), rabbit anti-myc antibody (1:1000) (14793S), rabbit anti-p53 (7F5) (1:1000)(2527S), rabbit anti-E-Cadherin (24E10) (1:1000)(3195S), rabbit anti-N-Cadherin (D4R1H) XP®(1:1000)(13116S) and mouse anti-ubiquitin (1:1000) (#3936) were purchased from Cell Signalling Technology.
MG132 (HY-13259), Cycloheximide (CHX) (HY-12320) and Cell Counting Kit-8 (CCK-8, HY-K0301) were purchased from MedChemExpress (Shanghai, NJ, USA).

Cells were harvested at a density above 90% and subsequently lysed in RIPA buffer (Solarbio, China) on ice. Equal protein lysates (20 μg) were transferred to polyvinylidene fluoride membranes (Millipore, County Cork, Ireland) through sodium dodecyl sulfate polyacrylamide gels. Membranes were blocked in rapid block buffer (Sangon Biotech, China) for 15 min and then incubated at 4°C overnight with relevant primary antibodies. The Horse Radish Peroxidase-conjugated secondary antibodies were then used to incubate the membranes for 1 h at room temperature. Primary antibodies used were rabbit anti-MCM3 (dilution, 1:2000; cat. no., ab80044; Abcam), rabbit anti-CDK2 (dilution, 1:1000; cat. no., 2546; Cell Signaling), rabbit anti-CDK4 (dilution, 1:1000; cat. no., 12790; Cell Signaling), rabbit anti-Cyclin D1 (dilution, 1:1000; cat. no., 26939-1-AP; Proteintech), rabbit anti-Cyclin E1 (dilution, 1:1000; cat. no., 11554-1-AP; Proteintech), and rabbit anti-β-actin (dilution, 1:5000, cat. no., 20536-1-AP; Proteintech).