All the chemicals were of analytical grade and used as received. All solutions were prepared with ultrapure water (18 MΩcm) from a Millipore system. 1H NMR and 13C NMR were acquired in CDCl3 on BRUKER AVANCE 500 spectrometer using TMS as an internal standard. HRMS were obtained on HP5989 mass spectrometer. The dark-field spectrum measurements were carried out on an inverted microscope (eclipse Ti-U, Nikon, Japan) equipped with a dark field condenser (0.8 < NA < 0.95), a 100 W halogen lamp, a true-color digital camera (Nikon DS-fi), a monochromator (Acton SP2300i) equipped with a spectrograph CCD (CASCADE 512B, Roper Scientific) and a grating (grating density: 300 L/mm; blazed wavelength: 500 nm). The true-color scattering images of gold nanoparticles were taken using a 40X objective lens (NA = 0.8). The scattering spectra from the individual nanoparticles were corrected by subtracting the background spectra taken from the adjacent regions without the GNPs and dividing it with the calibrated response curve of the entire optical system. The spectra were integrated for 10 seconds.
Ds fi
Manufactured by Nikon
Sourced in Japan
The DS-fi is a digital microscope camera from Nikon. It is designed for capturing high-quality images of microscopic samples. The camera features a 5.0 megapixel CMOS sensor and supports a wide range of resolutions and frame rates. It can be connected to a computer for image acquisition and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Eclipse ti u, by Nikon (3 mentions)
Cascade 512b, by Teledyne (1 mentions)
Avance 500 spectrometer, by Bruker (1 mentions)
Smz800 stereo macroscope, by Nikon (1 mentions)
Acton sp2300i, by Teledyne (1 mentions)
Pixis 100bx, by Teledyne (1 mentions)
Az100, by Nikon (1 mentions)
Smz745t, by Nikon (1 mentions)
6 protocols using ds fi
1
Characterization of Gold Nanoparticles
2
Intestinal Sample Clearing Imaging
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Images illustrating the clearing steps of thick intestinal sample were obtained with the SMZ800 stereo macroscope, equipped with a digital camera DS-Fi and DS-U2/L2 USB (Nikon, Amstelveen, The Netherlands). Samples were illuminated with white light (3,000°K) and the images acquired with the NIS-Element software.
3
Plasmonic Nanoantenna Scattering Characterization
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The plasmon resonance scattering measurements were carried out on an inverted dark-field microscope (eclipse Ti-U, Nikon) using a ×40 objective lens (numerical aperture, 0.6) and a dark-field condenser (0.8 < numerical aperture < 0.95). A halogen lamp (100 W) was used as a source of white light to generate plasmon resonance scattering light. The dark-field images were captured by a true-colour digital camera (Nikon DS-fi). The light scattered from the bifunctionalized nanoantennae was split by a monochromator (grating density, 300 lines mm–1; blazed wavelength, 500 nm; Acton SP2300i, Princeton Instruments). An IsoPlane-320 spectrometer was used, and the split light was collected by a charge-coupled device (Pixis 100BX, Princeton Instruments). An a.c. EF of 3 MHz at 0.65 V was applied for 10 min, and scattering spectra were monitored (1,000 frames recorded). The exposure time was 500 ms. The samples for plasmon resonance scattering were prepared by immobilizing nanoantennae on ITO. First, ITO slides were treatment with ethanol, acetone and water under sonication. Next, 50 µl nanoantennae solution was drop-casted on the ITO slides for 10 min, followed by a single-step washing and rinsing with water. Finally, the slides were dried with N2 gas.
4
Dark-Field Imaging and Spectroscopy of Gold Nanoparticles
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As shown in Figure 1 A, the dark-field measurements were carried out on an inverted microscope (eclipse Ti-U, Nikon, Japan) that was equipped with a dark-field condenser (0.8 < NA < 0.95) and a 40× objective lens (NA = 0.8). The GNP-functionalized slides were immobilized on a platform, and the white light source (a 100 W halogen lamp) was used to excite the GNPs and generate plasmon resonance scattering light. A true-color digital camera (Nikon DS-fi, Japan) was used to capture the dark-field images. The scattering light of gold nanoparticle was split by a monochromator (ActonSP2300i, PI, USA) that was equipped with a grating (grating density: 300 lines/mm; blazed wavelength: 500nm) and recorded by a spectrometer CCD (pixis 400, PI, USA) to obtain the scattering spectra.
5
Amniote Taxon Histological Thin Section Preparation
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Histological thin sections of several amniote taxa (Additional file 1 : Table S1) were prepared by first embedding specimens in Castolite AP or Castolite AC polyester resin and placing them under vacuum. One specimen (MOR 559) was embedded in Buehler Epothin resin. Embedded materials were then cut using the Buehler Isomet slow-speed wafer blade saw and the cut surfaces were polished using 600-grit silicon carbide powder. For two specimens (MOR 548, 559), thin wafers were cut using a Buehler Isomet 1000 high-speed wafer blade saw. Specimens were later mounted to frosted plexiglass slides using cyanoacrylate and cut using the Isomet saw. Specimens were then ground down using a Hillquist or a Buehler Ecomet grinding machine and further polished using progressively finer grits of silicon carbide and aluminium oxide powders. The ROM thin sections were imaged using a Nikon DS-Fi camera mounted to a Nikon AZ-100 microscope with NIS Elements BR imaging software registered to D. C. Evans or R. R. Reisz.
6
Quantifying Rachis Brittleness in Wheat
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For the greenhouse experiment, rachis brittleness was assessed through mechanical processing of spikes in an adapted threshing machine equipped with cooking grade silicone toothed rotor blades. Spikes were threshed for five seconds at 900 rpm. The threshed material was collected in a removable plastic tray. Rachis fragility, in percentage, was calculated as previously reported by Komatsuda et al. (2004) (link), i.e., the percentage of rachis nodes disarticulated over the total number of rachis nodes in a spike, measured in five F 1 plants per genotype, using two spikes per plant, at two different times (2 and 4 weeks after ripening, determined as stage Z91 (Zadoks, Chang, & Konzak, 1974 (link))) (Video S1).
In the field nursery, all the spikes from three to ten plants of each genotype were bagged with breathable and translucent bags (Fito Agrícola S.L., Castellón, Spain).
Spontaneous spikelet disarticulation was measured, at three different times (two, three, and four weeks after Z91), through the counting of the number of disarticulated rachis nodes per number of spikes inside the bag.
In addition, the disarticulation scars from a representative sample of brittle and nonbrittle spikes were evaluated with the aid of a Nikon SMZ 745 T stereomicroscope connected to a Nikon DS-Fi camera.
In the field nursery, all the spikes from three to ten plants of each genotype were bagged with breathable and translucent bags (Fito Agrícola S.L., Castellón, Spain).
Spontaneous spikelet disarticulation was measured, at three different times (two, three, and four weeks after Z91), through the counting of the number of disarticulated rachis nodes per number of spikes inside the bag.
In addition, the disarticulation scars from a representative sample of brittle and nonbrittle spikes were evaluated with the aid of a Nikon SMZ 745 T stereomicroscope connected to a Nikon DS-Fi camera.
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