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Spin x uf concentrators

Manufactured by Merck Group
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The Spin-X® UF concentrators are a type of lab equipment used for the concentration and purification of macromolecules, such as proteins and nucleic acids, through the process of ultrafiltration. The device features a centrifugal design that facilitates the separation and concentration of the desired molecules from the sample solution.

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Lab products found in correlation

Alkaline phosphatase assay kit colorimetric, by Abcam (2 mentions) Fugene 6 reagent, by Promega (2 mentions)

2 protocols using spin x uf concentrators

1

Recombinant Semaphorin 4D Production

Cited 1 time
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Recombinant Semaphorin 4D was produced as a secreted protein fused with alkaline phosphatase (AP). To produce recombinant Semaphorin, human embryonic kidney HEK293T cells were transfected with Sema4D-AP in pcDNA3 vector 47 (link), using Fugene 6 reagent according to the manufacturer’s protocol (Promega) The supernatant of transfected cells was collected, filtered and concentrated 10 fold by Spin-X® UF concentrators with a 100Kd cut-off (Millipore). Protein concentration was normalized to a range of 3-6nM by measurement of the AP-activity using Alkaline Phosphatase Colorimetric Assay kit (Abcam).
Azzarelli R., Pacary E., Garg R., Garcez P., van den Berg D., Riou P., Ridley A.J., Friedel R.H., Parsons M, & Guillemot F. (2014). An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration. Nature Communications, 5, 3405.
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2

Production of Recombinant Semaphorin 4D

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant Semaphorin 4D was produced as a secreted protein fused with AP. To produce recombinant Semaphorin, human embryonic kidney HEK293T cells were transfected with Sema4D-AP in pcDNA3 vector47 (link), using Fugene 6 reagent according to the manufacturer’s protocol (Promega) The supernatant of transfected cells was collected, filtered and concentrated 10-fold by Spin-X UF concentrators with a 100kDa cutoff (Millipore). Protein concentration was normalized to a range of 3–6 nM by measurement of the AP-activity using Alkaline Phosphatase Colorimetric Assay kit (Abcam).
Azzarelli R., Pacary E., Garg R., Garcez P., van den Berg D., Riou P., Ridley A.J., Friedel R.H., Parsons M, & Guillemot F. (2014). An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration. Nature Communications, 5, 3405.
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