Human gastric cancer cell lines BGC823 and MGC803 were obtained from Cell Bank of Type Culture Collection (Shanghai, China). The cell lines were cultured using RPMI 1640 medium with 10% fetal bovine serum (FBS; Gibco, NY, USA) at 37 °C and 5% CO2. The synthetic mimics of miR-1252 were obtained from GenePharma (Shanghai, China). The sequence of circ_0000190 and PAK3 was obtained by PCR, and then inserted into pcDNA3.0 vectors (Invitrogen, CA, USA) after confirmation. As respective negative controls (NC), the scramble RNA and empty expression vectors were used in this study. The BGC-823 and MGC803 cells were cultured in 6-well plates for 24 h, and transfection was conducted according to the manufacturer’s protocol with lipofectamine 2000 (Invitrogen, CA, USA).
Synthetic mimics of mir 1252
Manufactured by GenePharma
Sourced in China
Synthetic mimics of miR-1252 are small, chemically synthesized RNA molecules designed to mimic the structure and function of the endogenous microRNA, miR-1252. These synthetic mimics can be used to study the biological role and potential applications of miR-1252 in various research and experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Mgc 803, by Thermo Fisher Scientific (3 mentions)
Bgc 823, by Thermo Fisher Scientific (3 mentions)
Pcdna3.0 vector, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Lipfectamine 2000, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
3 protocols using synthetic mimics of mir 1252
1
Gastric Cancer Cell Line Transfection
2
Gastric Cancer Cell Line Culturing and Transfection
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Human gastric cancer cell lines BGC823 and MGC803 were obtained from Cell Bank of Type Culture Collection (Shanghai, China). The cell lines were cultured using RPMI 1640 medium with 10% fetal bovine serum (FBS; Gibco, NY, USA) at 37°C and 5% CO 2 . The synthetic mimics of miR-1252 were obtained from GenePharma (Shanghai, China). The sequence of circ_0000190 and PAK3 was obtained by PCR, and then inserted into pcDNA3.0 vectors (Invitrogen, CA, USA) after con rmation. As respective negative controls (NC), the scramble RNA and empty expression vectors were used in this study. The BGC-823 and MGC803 cells were cultured in 6-well plates for 24h, and transfection was conducted according to the manufacturer's protocol with lipfectamine 2000 (Invitrogen, CA, USA).
3
Examining miR-1252 and circ_0000190 in Gastric Cancer
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Human gastric cancer cell lines, BGC823 and MGC803, were obtained from Cell Bank of Type Culture Collection (Shanghai, China). The cell lines were cultured using RPMI 1640 medium with 10% fetal bovine serum (FBS; Gibco, NY, USA) at 37°C and 5% CO2.The synthetic mimics of miR-1252 were obtained from GenePharma (Shanghai, China). The sequence of circ_0000190 and PAK3 was obtained by PCR, and then inserted into pcDNA3.0 vectors (Invitrogen, CA, USA) after con rmation. As respective negative controls (NC), the scrambled RNA and empty expression vectors were used in this study. The BGC-823 and MGC803 cells were cultured in 6-well plates for 24h, and transfection was conducted according to the manufacturer's protocol with lipfectamine 2000 (Invitrogen).
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