After centrifugation, the supernatant of 1 mL culture was directly used for NO3− concentration measurements on an ICS-6000 ion chromatography (IC) system (Thermo Fisher Scientific, Waltham, MA, USA). A Dionex IonPac AS11 with guard column (Thermo Fisher Scientific, Waltham, MA, USA) was set to 30 °C and operated with a flow of 2.0 mL·min−1. Mobile phase A was 100% H2O and phase B 100 mmol·L−1 NaOH. The binary gradient was from 0 to 1 min 0.2% B, from 1 to 6 min to 5% B, from 6 to 13 min increase to 24% B, from 13 to 13.5 min set to 38% B, and hold until 14.5 min. After this, the initial 0.2% B was reached after 15 min and held until 20 min for equilibration before the next injection. A conductivity detector combined with AERS suppressor (Thermo Fisher Scientific, Waltham, MA, USA) was used for analyte detection. Nitrate standards (Sigma Aldrich, St. Lewis, MO, USA) were prepared and used for quantification of the samples. Data was logged and evaluated by Chromeleon 7.2.8 (Thermo Fisher Scientific, Waltham, MA, USA).
Dionex ionpac as11
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Dionex IonPac AS11 is a high-performance anion-exchange chromatography column designed for the separation and analysis of a wide range of inorganic and organic anions. It features a silica-based stationary phase with a strongly basic quaternary ammonium functionality, providing efficient and reproducible separations.
Automatically generated - may contain errors
2 protocols using dionex ionpac as11
1
Nitrate Analysis via Ion Chromatography
2
Quantification of Cellular Lactate Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lactate in the cell medium was detected and quantified by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) using the Dionex ICS-4000 Ion Chromatography System (Thermo Scientific). After treatments, medium was removed from the cell monolayers and centrifuged at 1000g to remove cellular debris. Cellfree medium was deproteinized by adding an equal volume of ACN, incubating on ice for 5 min and centrifuging for 10 min at 18 000g at 4 °C. The sample was diluted 50-fold in MilliQ water to fall within the linear range of lactate detection (1-20 μg ml -1 ) and applied on the Dionex IonPac AS11 (Thermo Scientific) anion exchange column, equilibrated in 1 mM KOH, and a 1-60 mM KOH gradient was used for elution. A standard calibration curve was obtained from linear range of concentrations of ICE standard lactate (Sigma).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!