Ros detection kit s0033s

Iron(II) chloride tetrahydrate (FeCl₂·4H₂O), cobalt chloride hexahydrate (CoCl₂·6H₂O), manganese chloride tetrahydrate (MnCl₂·4H₂O), nickel chloride hexahydrate (NiCl₂·6H₂O), Sodium tungstate dihydrate (Na₂WO₄·2H₂O), hexadecyl trimethyl ammonium Bromide (CTAB), 1,3-diphenylisobenzofuran (DPBF) and 3,3,5,5-Tetramethylbenzidine (TMB) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All chemicals were of analytical grade and used without further purification.
The ROS detection kit (S0033S) and propidium iodide (PI) (ST511) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). The primary antibodies, Anti-GAPDH and anti-AID, were purchased from Abways Technology Co., Ltd. (Shanghai, China). and North American ImmunoWay Biotechnology Company (TX, USA). The ECL Substrate Kit (P0018FS) was obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China).
Human DLBCL SU-DHL-4 and OCI-LY19 cell lines were purchased from BeNa Culture Collection (BeNa, #BNCC340176, #BNCC338225) (Suzhou, China).

Cadmium chloride (CdCl₂, 202908), NAC (30498), and DFO (D9533) were obtained from Sigma-Aldrich (Sigma, St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 12800-017), fetal bovine serum (FBS, Gibco, 10437-028), Trizol (Ambion, 15596026), and trypsin-EDTA (25200072) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The ROS detection kit (S0033S), Mito-Tracker Red fluorescent probe (C1035), and Bicinchoninic acid (BCA) protein assay kit (P0012) were from Beyotime Biotechnology Co., Ltd. (Beyotime, Shanghai, China). The MDA (A003-1-2) and GSH detection kits (A006-2-1) were purchased from Nanjing Jiancheng Bio-Engineering Institute Co., Ltd. (Nanjing, China). The Cell Counting Kit-8 (CCK-8, A311-01) was purchased from Vazyme Biotechnology Co., Ltd. (Vazyme, Nanjing, China). The PrimeScrip^TM RT Reagent Kit (RR037A) and SYBR Green™ Premix Ex Taq™ (RR390A) were obtained from Takara Biomedical Technology Co., Ltd. (Takara, Beijing, China). The RIPA lysate (20101ES60) was obtained from New Cell & Molecule Biotechnology Co., Ltd. (NCM, Suzhou, China). The Fer-1 (HY-100579) and Erastin (HY-15763) were purchased from MedChemExpress LLC (MCE, Princeton, NJ, USA). All chemicals were of the highest purity grade available.