Trastuzumab (Herceptin®, Roche, binds HER2); pertuzumab (Perjeta®, Roche, binds HER2); TS2/16.2.1 mouse monoclonal produced in-house from hybridoma (ATCC Cat# HB-243, binds integrin β1); BIIG2 rat monoclonal produced in-house from hybridoma (DSHB Antibody Registry ID: AB_528155, binds α5 integrin); ab528 mouse monoclonal produced in-house from hybridoma (ATCC Cat# HB-8509 binds: epidermal growth factor receptor EGFR);Alexa Fluor 546 conjugated goat anti-Mouse IgG (H + L), highly cross-adsorbed (ThermoFisher cat# A-11030); Alexa Fluor 647 conjugated Goat anti-Mouse IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21235); Alexa Fluor 546 conjugated Goat anti-Human IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21089); Alexa Fluor 647 conjugated Goat anti-Human IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21445);Alexa Fluor 647 conjugated Goat anti-Rat IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21247).
Alexa fluor 647 conjugated goat anti rat igg h l
Manufactured by Thermo Fisher Scientific
Alexa Fluor 647 conjugated Goat anti-Rat IgG (H + L) is a secondary antibody used for the detection and quantification of rat immunoglobulins in various immunochemical applications, such as Western blotting, immunohistochemistry, and flow cytometry. The Alexa Fluor 647 dye provides far-red fluorescence emission, which can be detected using appropriate instrumentation.
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Lab products found in correlation
Alexa fluor 546 conjugated goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 conjugated goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 546 conjugated goat anti human igg, by Thermo Fisher Scientific (1 mentions)
Herceptin, by Roche (1 mentions)
Perjeta, by Roche (1 mentions)
Rat monoclonal anti cd31, by BD (1 mentions)
2 protocols using alexa fluor 647 conjugated goat anti rat igg h l
1
Multiparametric Imaging of Cell Surface Receptors
2
Whole-Mount Retina and Choroid Immunostaining
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Enucleated eyes from 8-week-old C57BL/6J mice were fixed for 30 minutes in 4% paraformaldehyde (PFA) in PBS and then dissected. Retinal cups were removed before immunostaining the whole-mount retina. To whole-mount immunostain the choroid, the RPE/choroid/sclera complex was additionally fixed with 4% PFA for 30 minutes, and the RPE was removed by gentle brushing, resulting in a choroid/sclera complex. The retinal cups and choroid/sclera complexes were stained with primary antibodies, including rat monoclonal anti-CD31 (1:200, BD, cat. #553370) and PE anti-mouse CD157 antibody (clone BP-3, BioLegend, cat. #140204). The secondary antibody used was Alexa Fluor 647-conjugated goat anti-Rat IgG (H+L) (1:500, Thermo Fisher, cat. #A21247).
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