Alexa fluor 546 conjugated anti mouse or anti rabbit igg

Rabbit polyclonal antibody (pAb) against Myc (R1208-1), GAPDH (glyceraldehyde-3-phosphate dehydrogenase; EM1101), and β-actin (EM21002) were purchased from Huaan Biological Technology (Hangzhou, China). Mouse anti-HA (H3663) and anti-Flag (F1804) MAbs were both obtained from Sigma-Aldrich (USA). Anti-Flag affinity resin (A2220) for immunoprecipitation was also purchased from Sigma-Aldrich. Rabbit pAb against ubiquitin (ab7780) were purchased from Abcam (USA). Rabbit anti-K63 (D7A11) and anti-K48 (D9D5) MAbs were obtained from Cell Signaling Technology (USA). Rabbit pAb to VP1 of IBDV, chicken pAb to VP3 of IBDV, and mouse MAb to VP1, VP2, VP3, VP4, and VP5 of IBDV were produced by our laboratory (17 (link), 51 (link), 52 (link)). MG132 (S2619) was purchased from Selleckchem (USA). Cycloheximide (CHX) was obtained from Medchemexpress (HY-12320). N-ethylmaleimide (NEM), a deubiquitination inhibitor, was obtained from Sigma-Aldrich (E3876). Cell NP-40 lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40) was purchased from Beyotime (P0013F; Shanghai, China). Horseradish peroxidase (HRP)- or fluorescein isothiocyanate (FITC)-labeled goat anti-mouse and anti-rabbit IgG were purchased from KPL (Milford, MA). FITC-labeled goat anti-chicken antibodies were purchased from Abcam (ab6873). Alexa Fluor 546-conjugated anti-mouse or anti-rabbit IgG were obtained from Invitrogen (USA).

Rabbit polyclonal antibodies (pAb) against Myc (R1208-1), GFP (SR48-02), FLAG (091 (link)2-1), and mouse mAbs against β-actin (M1210-2) and GST (M0807-1) were purchased from Huaan Biological Technology (Hangzhou, China). Rabbit pAbs against histone H3 (R1105-1) and β-tubulin (0807-2 (link)) were also purchased from Huaan Biological Technology. Mouse anti-Myc (05-419) and anti-FLAG (F1804) mAbs were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-FLAG affinity resin (A2220) for immunoprecipitation was also purchased from Sigma-Aldrich. Rabbit mAb against NPM1 (ab52644) was purchased from Abcam (Cambridge, MA). Mouse mAbs to Cap, Rep, ORF3, and ORF4 of PCV2 were produced by our laboratory [24 (link),36–38 (link)]. NP-40 cell lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40) was purchased from Beyotime (P0013F; Shanghai, China). Horseradish peroxidase (HRP) or fluorescein isothiocyanate (FITC)-labeled goat anti-mouse and anti-rabbit IgG were purchased from KPL (Milford, MA, USA). Alexa Fluor 546-conjugated anti-mouse or anti-rabbit IgG were obtained from Invitrogen (USA).