Lactate and glucose were measured in blood using an Accutrend analyzer (Roche). Plasma Triacylglyceride (TAG) levels were determined by an enzymatic kit from Biosystem (Barcelona, Spain). NEFA and ketone bodies (KB) were measured with enzymatic kits from WAKO Chemical (Neuss, Germany). Plasma leptin and insulin levels were assayed using specific rat ELISA kits from SPI-Bio (Montigny le Bretonneux, France). Plasma resistin was assessed using a rat resistin ELISA kit (BioVendor, Brno, Czech Republic), following the manufacturer’s instructions. Cytokines were analysed using MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel, and Immunology Multiplex Assay form Merk-Millipore (Darmstadt, Germany). Homeostasis model assessment for insulin resistance (HOMA-IR) was calculated as fasting insulin (µU/ml) × fasting glucose ([mmol/liter]/22.5) as described earlier41 (link).
Rat resistin elisa kit
Manufactured by BioVendor
Sourced in Czechia
The Rat Resistin ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of rat resistin levels in serum, plasma, and cell culture supernatants. The kit utilizes a specific antibody coated on a 96-well plate to capture the target analyte, and a detection antibody coupled with an enzymatic readout system to quantify the captured analyte.
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2 protocols using rat resistin elisa kit
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Metabolic Biomarker Profiling in Rats
2
Metabolic Biomarkers Determination
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Serum determinations were measured as previously described [40] . Triacylglycerides (TG) were determined by enzymatic kits from Biosystem (Barcelona, Spain). Nonesterified fatty acid (NEFA) levels were measured with an enzymatic assay from Wako Chemicals (Neuss, Germany) and glucose levels were determined using AmplexRed glucose/Glucose Oxidase Assay Kit (Molecular Probes, Inc., Eugene, OR). Serum insulin and leptin levels were measured by ELISA using rat specific kits from SPI-Bio (Montigny le Bretonneux, France). Serum resistin was assessed using a rat resistin ELISA kit (BioVendor, Brno, Czech Republic), following the manufacturer's instructions. Lee index was calculated as described previously [36] .
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