Protein samples were separated with a 4-12% Bis-Tris NuPAGE gel (Invitrogen) and transferred onto nitrocellulose membrane by the Trans-blot SD semi-dry transfer cell (Bio-Rad, Brea, CA, USA). The membranes were blocked in TBST buffer containing 5% nonfat milk (Santa Cruz Biotech), and incubated with rabbit antibody against human SOX4 at a dilution of 1:500 (Abcam) overnight, followed by HRP linked goat anti-rabbit IgG (1:5000; GE Healthcare). The detection was performed with the ECL-Plus Western blotting reagent kit (GE Healthcare).
Ecl plus western blotting reagent kit
Manufactured by GE Healthcare
Sourced in United States
The ECL-Plus Western blotting reagent kit is a laboratory equipment product designed for the detection and visualization of proteins in Western blot analysis. It provides a chemiluminescent substrate that enables the detection of target proteins labeled with enzyme-conjugated antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Nupage bis tris gel, by Thermo Fisher Scientific (6 mentions)
Trans blot sd semi dry transfer cell, by Bio-Rad (6 mentions)
Non fat milk, by Santa Cruz Biotechnology (3 mentions)
Anti grp78, by Santa Cruz Biotechnology (1 mentions)
Hrp linked secondary antibody, by GE Healthcare (1 mentions)
Non fat dry milk, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated goat anti rabbit igg, by Abcam (1 mentions)
Hrp linked sheep anti mouse igg, by GE Healthcare (1 mentions)
Horseradish peroxidase hrp linked secondary antibody, by GE Healthcare (1 mentions)
8 protocols using ecl plus western blotting reagent kit
1
Western Blot Analysis of SOX4 Protein
2
Protein Expression Analysis by Western Blot
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Protein samples were separated with a 4-12% Bis-Tris NuPAGE gel (Invitrogen) and transferred onto nitrocellulose membrane by the Trans-blot SD semi-dry transfer cell (Bio-Rad, Brea, CA, USA). The membranes were blocked in TBST buffer containing 5% nonfat milk (Santa Cruz Biotech), and incubated with antibodies against human protein (anti-GRP78, anti-CHOP, anti-IRE1a, anti-XBP1, anti-ATF4 and anti-ATF6a) at a dilution of 1:500 (Santa Cruz Biotech) overnight, followed by HRP linked anti-mouse or anti-rabbit IgG (1:5000; GE Healthcare). The detection was performed with the ECL-Plus Western blotting reagent kit (GE Healthcare). Western blot analysis was performed in triplicates.
3
Quantifying CD36 Protein Expression
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Equal amount of protein samples was loaded and separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel (Invitrogen). Then, the blots on the gels were transferred onto a nitrocellulose membrane. Follow by blocking with 5% non-fat milk in Tris buffered saline with Tween-20 (TBST), the membranes were probed with CD36 primary antibody (Santa Cruz Biotechnology, Inc, Dallas, Texas) overnight at 4°C. Then the membranes were washed with TBST for 3 times and incubated with secondary antibody at room temperature for 1 hour. The signal was detected using ECL-Plus Western blotting reagent kit (GE Healthcare Chicago, Illinois).
4
Western Blot Protein Analysis
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Protein samples were separated with a 4-12% Bis-Tris NuPAGE gel (Invitrogen) and transferred onto nitrocellulose membrane by Trans-blot SD semi-dry transfer cell (Bio-Rad). After saturating with 5% milk in Tris Buffered Saline-Tween (TBST) buffer, the membranes were sequentially incubated with primary antibodies (Santa Cruz Biotech) overnight at 4°C and HRP-linked secondary antibodies, respectively (GE Healthcare). The detection was performed with the ECL-Plus Western blotting reagent kit (GE Healthcare).
5
Western Blot Analysis of TKT and AK2
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Protein samples were separated with a 4-12% Bis-Tris NuPAGE gel (Invitrogen, Carlsbad, CA, USA) and transferred onto nitrocellulose membrane by Trans-blot SD semi-dry transfer cell (Bio-Rad, Hercules, CA, USA). The membrane was then blocked with 5% non-fat dry milk (Santa Cruz, USA) for one hour, and then sequentially incubated with anti-TKT (H50, sc-67120) or anti-AK2 (H65, sc-28786) primary antibody (Santa Cruz Biotech, Santa Cruz, CA, USA) and corresponding secondary antibody (GE Healthcare, Piscataway, NJ, USA). The detection was performed with the ECL-Plus Western blotting reagent kit (GE Healthcare).
6
Western Blot Analysis of ZNF569 Protein
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The protein samples were loaded and separated on a 4-12% Bis-Tris NuPAGE gel (Invitrogen) and transferred onto a nitrocellulose membrane at 120V for 1.5 h. The membranes were blocked in TBST buffer containing 5% nonfat milk at room temperature for 1h, and incubated with rabbit antibody against human ZNF569 at a dilution of 1:150 (Abcam, Cambridge, UK) overnight in the cold room, followed by HRP conjugated goat anti-rabbit IgG (1:5000; Abcam) at room temperature for 1h. Signal detection was performed with the ECL-Plus Western blotting reagent kit (GE Healthcare, Piscataway, NJ, USA).
7
Syntenin-1 Protein Detection by Western Blot
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The protein samples were loaded and separated on a 4–12% Bis-Tris NuPAGE gel (Invitrogen) and transferred onto a nitrocellulose membrane using a Trans-blot SD semi-dry transfer cell (Bio-Rad). The membranes were blocked for 2 h at room temperature in TBST buffer containing 5% nonfat milk (Santa Cruz Biotech), and incubated with mouse antibody against human Syntenin-1 (1:500; Santa Cruz Biotech) overnight, followed by HRP-linked sheep anti-mouse IgG (1:5000; GE Healthcare). Signal detection was performed with the ECL-Plus Western blotting reagent kit (GE Healthcare).
8
Western Blot Analysis of C5AR1 Protein
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The proteins extracted from the cell samples were separated on a 4%-12% Bis-Tris NuPAGE gel and transferred onto a polyvinylidene difluoride membrane using a Trans-blot SD semidry transfer cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated with primary C5AR1 antibody (1 : 100; Santa Cruz Biotechnology) overnight, followed by horseradish peroxidase- (HRP-) linked secondary antibody (1 : 5000; GE Healthcare, Piscataway, NJ, USA). Signal detection was performed with the ECL-Plus western blotting reagent kit (GE Healthcare).
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