Seongsanamide A (>98%, Figure 1 ) was isolated from the fermentation broth extracts of Bacillus sp. KCTC 12796BP by as previously reported [7 (link)]. Acetaminophen, amodiaquine, bupropion, chlorzoxazone, dextromethorphan, trimipramine, uridine diphosphoglucuronic acid (UDPGA), nicotinamide adenine dinucleotide phosphate (NADP+), glucose-6-phosphate (G6P), and G6P dehydrogenase (G6PDH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Coumarin, midazolam, omeprazole, and tolbutamide were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Pooled HLMs (XTreme 200) were supplied by XenoTech (Lenexa, KS, USA). We purchased rP450 isoforms (rCYP1A2, rCYP2A6, rCYP2B6, rCYP2C8, rCYP2C9, rCYP2C19, rCYP2D6, rCYP2E1, rCYP2J2, rCYP3A4, and rCYP3A5) from Corning life sciences (Woburn, MA, USA). All solvents used in the analyses were LC-MS grade (Fisher Scientific Co., Pittsburgh, PA, USA).
G6p dehydrogenase g6pdh
Manufactured by Merck Group
Sourced in United States
G6P dehydrogenase (G6PDH) is an enzyme that catalyzes the first step in the pentose phosphate pathway. It converts glucose-6-phosphate to 6-phosphoglucono-delta-lactone, reducing NADP+ to NADPH in the process. The core function of G6PDH is to provide reducing power in the form of NADPH, which is essential for various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Glucose 6 phosphate (g6p), by Merck Group (2 mentions)
Acetaminophen, by Merck Group (1 mentions)
Chlorzoxazone, by Merck Group (1 mentions)
Dextromethorphan, by Merck Group (1 mentions)
Tolbutamide, by LGC (1 mentions)
Lc ms grade, by Thermo Fisher Scientific (1 mentions)
Bupropion, by Merck Group (1 mentions)
Trimipramine, by Merck Group (1 mentions)
Omeprazole, by LGC (1 mentions)
Midazolam, by LGC (1 mentions)
Coumarin, by LGC (1 mentions)
Pooled hlms xtreme 200, by Xenotech (1 mentions)
Amodiaquine, by Merck Group (1 mentions)
Resazurin, by Merck Group (1 mentions)
γ globulin, by Abcam (1 mentions)
Diaphorase, by Merck Group (1 mentions)
Phosphatase inhibitor cocktail a, by Beyotime (1 mentions)
Flavin mononucleotide, by Merck Group (1 mentions)
2 protocols using g6p dehydrogenase g6pdh
1
Seongsanamide A Biosynthesis and Assay
2
Biochemical Assay Protocol for NAADP
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NAADP was purchased from Tocris Bioscience. NADP was purchased from Abcam.
γ-globulin, PEG-8000, Flavin mononucleotide (FMN), resazurin, Glucose-6phosphate (G6P), G6P dehydrogenase (G6PDH) and Diaphorase were purchased from Sigma. Phosphatase inhibitor cocktail A was purchased from Beyotime.
γ-globulin, PEG-8000, Flavin mononucleotide (FMN), resazurin, Glucose-6phosphate (G6P), G6P dehydrogenase (G6PDH) and Diaphorase were purchased from Sigma. Phosphatase inhibitor cocktail A was purchased from Beyotime.
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