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Eclipse 80i microscope

Manufactured by Teledyne

The Eclipse 80i microscope is a high-performance optical microscope designed for a wide range of applications. It features a modular design, allowing for customization to meet specific user requirements. The microscope offers superior optical performance, providing clear and detailed images for precise observation and analysis.

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3 protocols using eclipse 80i microscope

1

Fluorescence Imaging of Cultured Cells

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Cells from 24 to 48h liquid culture were imaged using a Nikon Eclipse 80i microscope equipped with a Photometrics Cool Snap ES CCD camera (Roper Scientific). Illumination was provided by a metal halide light source (X-Cite). PBS fluorescence was detected using a 560/40 nm excitation filter, a 595 nm dichroic beam splitter, and a 630/60 nm emission filter.
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2

Immobilized Cell Microscopy Protocol

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Cells were immobilized on agarose pads (1% w/v in reverse osmosis-purified water). Microscopy was performed using a Nikon Eclipse 80i microscope with a PlanApo 100×, 1.40 NA objective and a Cascade 512B camera (Roper Scientific). Images were acquired using Metavue software (Universal Imaging).
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3

Measuring Cyanobacterial Heterocyst PBS

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Cells from 24-48h liquid culture were imaged using a Nikon Eclipse 80i microscope equipped with a Photometrics Cool Snap ES CCD camera (Roper Scientific).
Illumination was provided by a metal halide light source (X-Cite). PBS fluorescence was detected using a 560/40nm excitation filter, a 595nm dichroic beam splitter, and a 630/60nm emission filter.
Measuring PBS in heterocysts.
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