Vetbutal

Manufactured by Biowet

Sourced in Poland

Vetbutal is a laboratory equipment product that serves as a general anesthetic for veterinary use. It is designed to provide safe and effective sedation for animals during medical procedures or other situations requiring anesthesia. Vetbutal's core function is to induce a state of unconsciousness and pain relief in animals.

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Lab products found in correlation

Desipramine, by Merck Group (2 mentions) Rtv 3140, by Dow (1 mentions) Microm hm 560, by Zeiss (1 mentions) Sedazin, by Biowet (1 mentions) 6 ohda hbr, by Merck Group (1 mentions) Stereotaxic instrument, by Stoelting (1 mentions) Stereotactic apparatus, by Kopf Instruments (1 mentions) 6 hydroxydopamine hcl, by Merck Group (1 mentions) Model 5000, by Kopf Instruments (1 mentions) 6 ohda, by Merck Group (1 mentions) Ketamina 10, by Biowet (1 mentions) 4 0 monosyn, by B. Braun (1 mentions)

9 protocols using vetbutal

Vagus Nerve Stimulation in Wistar Rats

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Research was carried out on 12 male Wistar rats. All the animals were subjected to surgical implantation of a microchip in the abdominal region of the left vagus nerve. The animals were divided into two groups. The first one (MC, n = 6) underwent microchip stimulation of the left vagus nerve, while the control group (C, n = 6) was subjected to sham laparotomy.
For the purpose of surgical laparotomy, the subdiaphragmatic part of the left vagus nerve was connected to a 1-cm-diameter silicon-coated (RTV 3140, Dow Corning), battery-driven microchip. Experiments were carried out after 12 h of food deprivation, an empty stomach making it easier to access the nerve following the administration of pentobarbital (vetbutal, dose: 25 mg/kg of body mass; Biowet, Puławy, Poland) intraperitoneally. Once the abdominal part of the left vagus nerve was localized and electrically connected and the wounds were closed, the rats were moved to cages and subjected to 7 days of stimulation. Food and water were allowed ad libitum during the whole experiment. The period of the stimulating signal was 20 s, its duration was 0.1 s, and the amplitude was 200 mV. Finally, following electric stimulation, all the rats were guillotined. All procedures involving animals were approved by the Jagiellonian University Bioethical Committee (20/11/2009).

Surowka A.D., Krygowska-Wajs A., Ziomber A., Thor P., Chrobak A.A, & Szczerbowska-Boruchowska M. (2015). Peripheral Vagus Nerve Stimulation Significantly Affects Lipid Composition and Protein Secondary Structure Within Dopamine-Related Brain Regions in Rats. Neuromolecular Medicine, 17(2), 178-191.

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Transcardial Perfusion and Cryosectioning

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Six weeks after streptozotocin injection, animals were deeply anesthetized via intravenous administration of pentobarbital (Vetbutal, Biowet, Poland) and perfused transcardially via the ascending aorta with 4% paraformaldehyde in a 0.1 mol/L phosphate buffer (PB, pH 7.4). The samples were post-fixed by immersion in the same fixative for 1 h, rinsed several times with phosphatase buffer (PB) and then transferred into 30% sucrose solution and stored at 4 °C until sectioning. The tissue blocks were cut in frontal or sagittal planes using a Microm HM 560 cryostat (Carl Zeiss, Germany) at a thickness of 12 μm and mounted on gelatinized glass slides.

Bulc M., Palus K., Zielonka Ł., Gajęcka M, & Całka J. (2017). Changes in expression of inhibitory substances in the intramural neurons of the stomach following streptozotocin- induced diabetes in the pig. World Journal of Gastroenterology, 23(33), 6088-6099.

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Stereotaxic Surgery Protocol for Rats

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Standard stereotaxic surgery was performed under pentobarbital anesthesia (60 mg/kg i.p.) (Vetbutal, Biowet Puławy, Poland) with a premedication of xylazine (5 mg/kg i.p.) (Sedazin, Biowet, Puławy) and 0.1% solution of atropine (0.25 mg per animal) according to Myślińska and coworkers [25 (link)].

Karnia M.J., Myslinska D., Dzik K.P., Flis D.J., Ciepielewski Z.M., Podlacha M, & Kaczor J.J. (2018). The Electrical Stimulation of the Bed Nucleus of the Stria Terminalis Causes Oxidative Stress in Skeletal Muscle of Rats. Oxidative Medicine and Cellular Longevity, 2018, 4671213.

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Measuring Pancreatic Blood Flow in Rats

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Following 5-h infusion of saline or cerulein, the rats were anesthetized with pentobarbital (30 mg/kg i.p., Vetbutal, Biowet, Puławy, Poland) and then the abdominal cavity was opened. Pancreatic blood flow was measured by a laser Doppler flowmeter using a Laserflo, model BPM 403 A (Blood Perfusion Monitor, Vasdamedics Inc., St. Paul, MN, USA) as previously described [107 (link)]. Blood flow was measured in five different pancreatic regions in each rat and expressed as the percent change of the control value.

Bonior J., Warzecha Z., Ceranowicz P., Gajdosz R., Pierzchalski P., Kot M., Leja-Szpak A., Nawrot-Porąbka K., Link-Lenczowski P., Pędziwiatr M., Olszanecki R., Bartuś K., Trąbka R., Kuśnierz-Cabala B., Dembiński A, & Jaworek J. (2017). Capsaicin-Sensitive Sensory Nerves Are Necessary for the Protective Effect of Ghrelin in Cerulein-Induced Acute Pancreatitis in Rats. International Journal of Molecular Sciences, 18(7), 1402.

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Establishing 6-OHDA-Induced Parkinson's Model

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Under the pentobarbital anaesthesia (Vetbutal, Biowet, Poland; 25 mg/kg, ip) the animals were fixed into the stereotaxic instrument (Stoelting, USA) and injected bilaterally with 6-OHDA HBr [Sigma, Aldrich, 15 μg (free base) /2.5 μl per side, dissolved in 0.2% ascorbic acid] into the ventrolateral region of the caudate-putamen (AP: 1.2 mm, L: ± 3.1 mm, V: 6.8–7.0 mm from bregma according to Paxinos and Watson atlas [45 (link)]. Sham-operated rats which received 2.5 μl of 0.2% ascorbic acid bilaterally into the above region served as controls in all experiments. The injection cannulae were left in place for 60 s to enable full absorption of the solution. In order to spare noradrenergic terminals, desipramine (Sigma, Aldrich) was administered in a dose of 15 mg/2ml/kg ip 30 min before 6-OHDA injections. To avoid infections, the rats received an antibiotic (Lincospectin, Pharmacia, Belgium) 24 h before the operation, on the day of operation and 24 h afterwards.

Berghauzen-Maciejewska K., Wardas J., Kosmowska B., Głowacka U., Kuter K, & Ossowska K. (2015). Alterations of BDNF and trkB mRNA Expression in the 6-Hydroxydopamine-Induced Model of Preclinical Stages of Parkinson’s Disease: An Influence of Chronic Pramipexole in Rats. PLoS ONE, 10(3), e0117698.

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Mapping Somatosensory Cortical Activity

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2DG mapping was performed 24 hours after the last conditioning session. Mice were placed in the restraining holder and all vibrissae except row B on both sides of the snout were trimmed. Mice received injections of [¹⁴C]2-deoxyglucose (American Radiolabeled Chemicals, ARC, specific activity 55 mCi/mmol; 50 μCi/ 100 g body weight, i.m.) and the remaining row B vibrissae were stimulated with a mechanical stimulator at a frequency of 2 Hz for 30 min. Afterwards, mice received a lethal dose (0.2 ml/mouse) of barbiturate (Vetbutal, Biowet, Pulawy, Poland) and were briefly perfused transcardially with phosphate-buffered saline (PBS, pH 7.4) and 4% paraformaldehyde. The brains were removed and the cortex from two hemispheres was isolated, flattened between two glass slides [18 (link)], and fast frozen in heptane chilled to -60°C. The flattened cortex was cut at the cryostat into sections (30 μm) tangential to the cortex surface. Autoradiograms of 2DG labeling on serial brain sections and [¹⁴C] standards were obtained on Kodak mammography X-ray film after two weeks exposure. After obtaining 2DG autoradiograms, brain sections were counterstained with cresyl violet in order to identify barrels in layer IV of the somatosensory cortex.

Posluszny A., Liguz-Lecznar M., Turzynska D., Zakrzewska R., Bielecki M, & Kossut M. (2015). Learning-Dependent Plasticity of the Barrel Cortex Is Impaired by Restricting GABA-Ergic Transmission. PLoS ONE, 10(12), e0144415.

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6-OHDA Rat Model of Parkinson's Disease

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Animals were fully anaesthetized with pentobarbital anesthesia (50 mg/kg, i.p., Vetbutal, Biowet Pulawy, Poland) and atropine sulfate (0.25 mg/kg, s.c, Polfa, Poland) and placed in a stereotactic apparatus (Kopf Instruments, USA). The skull was exposed by a midline incision of the skin and a hole was drilled above the lesion site. The neurotoxin 6-OHDA (6-hydroxydopamine HCl, Sigma–Aldrich, Poland) was injected into the right SNpc in a volume of 4 μl (3 μg/μl dissolved in 0.9% NaCl containing 0.1% ascorbic acid). The following coordinates from the rat stereotactic atlas (Paxinos and Watson 2007 ) were used (in reference to bregma): anteroposterior (AP) – 5.3, lateral (L) – 2.4, dorsoventral (DV) – 7.5. Injections were performed using a microsyringe with a 26-gauge needle (Hamilton, USA) that was attached to a microinjection unit (Model 5000, Kopf Instruments, USA). The injection rate was 0.5 μl/min and the cannula was left in place for additional 5 min after injection to allow for diffusion into the tissue. To protect noradrenergic neurons from damage, animals received an intraperitoneal injection with the noradrenaline reuptake inhibitor desipramine (25 mg/kg, Sigma-Aldrich, Poland) 30 min prior to neurotoxin injection (Fulceri et al. 2006 (link)). Sham-lesioned controls underwent the same procedure but received vehicle instead of 6-OHDA.

Grembecka B., Glac W., Listowska M., Jerzemowska G., Plucińska K., Majkutewicz I., Badtke P, & Wrona D. (2020). Subthalamic Deep Brain Stimulation Affects Plasma Corticosterone Concentration and Peripheral Immunity Changes in Rat Model of Parkinson’s Disease. Journal of Neuroimmune Pharmacology, 16(2), 454-469.

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Toxin-Induced Murine Blood Analysis

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A day after the last toxin application, mice were placed in a state of deep anaesthesia for sacrifice by administration of Vetbutal (35 mg/kg bw; Biowet, Poland), and fasting whole blood samples were taken from the carotid artery. Then, whole blood was processed, and plasma ALP, ALT, AST, total protein (TP), triglyceride (TG), Ca and phosphorus (P) were measured using commercially available kits (Stamar, Dąbrowa Gornicza, Poland). Blood GSH levels were determined by Ellman’s method [15 (link)].

Martiniakova M., Sarocka A., Kovacova V., Kapusta E., Goc Z., Gren A., Formicki G, & Omelka R. (2020). Antagonistic Impact of Acrylamide and Ethanol on Biochemical and Morphological Parameters Consistent with Bone Health in Mice. Animals : an Open Access Journal from MDPI, 10(10), 1835.

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Dental Implant Osseointegration in Minipigs

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The study was performed on 12 16-month-old minipigs with an average weight of 50-60 kg. The study protocol was approved by the commission for animal studies.
The pigs underwent the removal of the permanent premolar teeth 2 months after the extraction of the deciduous premolar teeth in the jaw. Care was taken to avoid bone wall fractures. The extractions, implantations and euthanasia were performed under general anesthesia by intravenous application of pentobarbital (Vetbutal ® , Biowet Puławy, Poland) at doses of 25 mg/kg along with the premedication ketamine (Ketamina 10%, Biowet Puławy, Poland) and local infiltration of 2% lignocaine with noradrenaline 1:200,000 (Polfa, Poland). Eight weeks after the extractions, a total of 60 implants were inserted in the 12 animals. Each pig was to receive 5 implants (1 per surface state and 2 reference implants) in the mandible (Fig. 1,2). The position of the implants was statistically fully randomized. The implants were placed endosseously under continuous water cooling. After the insertion, the flap was repositioned using resorbable sutures (4/0 Monosyn ® , B. Braun, Melsungen, Germany). The animals were euthanized 12 weeks post-implantation using 50 mg/kg of pentobarbital (Morbital) and block biopsies of the implant sites were collected.

Kubasiewicz-Ross P., Hadzik J, & Dominiak M. (2018). Osseointegration of zirconia implants with 3 varying surface textures and a titanium implant: A histological and micro-CT study. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 27(9).

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