Serum was collected from clotted peripheral blood collected at each time point by venepuncture into silica containing vacutainers (Becton–Dickinson, Oxford, UK). Serum levels of Interleukin-6 (IL-6), IL-10, monocyte chemotactic protein-1 (MCP-1), brain-derived neurotrophic factor (BDNF) and soluble TNF receptor II (sTNFRII) were measured by Quantikine high sensitivity ELISAs (R&D systems, Minneapolis, USA). Vascular endothelial growth factor (VEGF) was measured using Duoset antibodies (R&D systems, Minneapolis, USA) according to manufacturer’s instructions. Haemoglobin levels were recorded separately as part of a participant’s clinical assessment by the clinical team.
Duoset antibodies
Manufactured by R&D Systems
Sourced in United States
The Duoset antibodies from R&D Systems are a set of primary and secondary detection antibodies designed for use in sandwich ELISA assays. They provide the necessary components to build custom ELISA kits to quantify target analytes in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using duoset antibodies
1
Cytokine and Growth Factor Profiling
2
Quantifying Activin A Levels
3
Quantifying Activin A Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Concentrations of activin A were determined using R&D Systems Duoset antibodies (Minneapolis, MN, USA).
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