Rna clean up

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The RNA clean-up product is designed to efficiently purify RNA samples from various sources. It removes contaminants and inhibitors, ensuring high-quality RNA suitable for downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit reticulocyte lysate, by Promega (2 mentions) Restriction enzymes, by Roche (2 mentions) Endoglycosidase h, by Roche (2 mentions) Pcr purification kit, by Thermo Fisher Scientific (2 mentions) Plasmid pgem1, by Promega (2 mentions) Proteinase k, by Merck Group (2 mentions) Tnt t7 quick coupled system, by Promega (1 mentions) Easytag express35s protein labeling mix, by PerkinElmer (1 mentions)

Spelling variants of this product

GeneJET RNA Clean-up Kit (3 citations)

2 protocols using rna clean up

In Vitro Protein Synthesis and Labeling

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All enzymes, as well as plasmid pGEM1, TNT T7 Quick Coupled System and rabbit reticulocyte lysate were from Promega (Madison, WI, USA). ER rough microsomes from dog pancreas were from tRNA Probes (College Station, TX, USA). EasyTag™ EXPRESS³⁵S Protein Labeling Mix, [³⁵S]-L-methionine and ³⁵S-L-cysteine, for in vitro labeling was purchased from Perkin Elmer (Waltham, MA, USA). Restriction enzymes and Endoglycosidase H were from Roche Molecular Biochemicals (Basel, Switerland). Proteinase K was from Sigma-Aldrich (St Louis, MO). The DNA plasmid, RNA clean-up and PCR purification kits were from Thermo Fisher Scientific (Ulm, Germany). All oligonucleotides were purchased from Macrogen (Seoul, South Korea).

Bañó-Polo M., Baeza-Delgado C., Tamborero S., Hazel A., Grau B., Nilsson I., Whitley P., Gumbart J.C., von Heijne G, & Mingarro I. (2018). Transmembrane but not soluble helices fold inside the ribosome tunnel. Nature Communications, 9, 5246.

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In Vitro Protein Translation Assay

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All enzymes, as well as plasmid pGEM1, RiboMAX SP6 RNA polymerase system and rabbit reticulocyte lysate were from Promega (Madison, WI). ER rough microsomes from dog pancreas were from tRNA Probes (College Station, TX, USA). [ 35 S]Met and [ 14 C]-methylated markers were from Perkin Elmer. Restriction enzymes and Endoglycosidase H were from Roche Molecular Biochemicals. Proteinase K was from Sigma-Aldrich (St Louis, MO). The DNA plasmid, RNA clean up and PCR purification kits were from Thermo Fisher Scientific (Ulm, Germany). All oligonucleotides were purchased from IDT (USA).

Bañó-Polo M., Martínez-Garay C.A., Grau B., Martínez-Gil L, & Mingarro I. (2017). Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit. Biochimica et biophysica acta. Biomembranes, 1859(5).

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