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β tubulin t7816

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β-tubulin (T7816) is a purified protein that can be used as a laboratory reagent. It is a component of the cytoskeleton and plays a role in cellular processes such as cell division and intracellular transport.

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Lab products found in correlation

β catenin 610153, by BD (1 mentions) Irdye 680lt goat anti rabbit igg, by LI COR (1 mentions) Gapdh g8795, by Merck Group (1 mentions) S1579, by Selleck Chemicals (1 mentions) Src 2109, by Cell Signaling Technology (1 mentions) Palbociclib, by Selleck Chemicals (1 mentions) Actin, by Cell Signaling Technology (1 mentions) Actin, by Santa Cruz Biotechnology (1 mentions) Rad51, by Cell Signaling Technology (1 mentions) Lc3b 2, by Cell Signaling Technology (1 mentions) Gamma h2ax, by Cell Signaling Technology (1 mentions) Total h2ax, by Cell Signaling Technology (1 mentions) Brca1, by Cell Signaling Technology (1 mentions) Complete mini protease inhibitor mixture tablet, by Roche (1 mentions) Actin sc 47778, by Santa Cruz Biotechnology (1 mentions)

3 protocols using β tubulin t7816

1

Western Blot Antibody and Chemical Reagents

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The CDK4 (A304-225A) antibody was purchased from Bethyl Laboratories (Montgomery, TX). AXL (4566), CAMKII (4436), CDK6 (13331), MEK1/2 (8727), MET (8198), MERTK (4319), and SRC (2109) were obtained from Cell Signaling Technology (Danvers, MA). β-catenin (610153) and GSK3β (610201) antibodies were purchased from BD Biosciences (San Jose, CA). GAPDH (G8795) and β-tubulin (T7816) antibodies were obtained from Sigma-Aldrich (St. Louis, MO). Secondary antibodies were purchased from LI-COR Biosciences (Lincoln, NE): IRDye® 800CW Goat anti-Mouse IgG (925-32210), IRDye® 680LT Goat anti-Mouse IgG (925-68020), IRDye®800CW Goat anti-Rabbit IgG (925-32211), and IRDye® 680LT Goat anti-Rabbit IgG (925-68021). The following chemicals were purchased from Cayman Chemicals (Ann Arbor, MI): abemaciclib (17740), CHIR-99021 (13122), dasatinib (11498), and ribociclib (17666). BMS-777607 (S1561) and palbociclib (S1579) were ordered from Selleck Chemicals (Houston, TX). Recombinant Wnt3A (315-20) was purchased from PeproTech (Rocky Hill, NJ).
Cousins E.M., Goldfarb D., Yan F., Roques J., Darr D., Johnson G.L, & Major M.B. (2017). Competitive Kinase Enrichment Proteomics Reveals that Abemaciclib Inhibits GSK3β and Activates WNT Signaling. Molecular cancer research : MCR, 16(2), 333-344.
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2

Protein Isolation and Analysis Protocol

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Total protein (~20 μg) was isolated from cells following 72 h treatment or vehicle control (0.02% DMSO or ethanol) for protein analysis as previously describe [40 (link),41 (link)]. Tris-glycine gels were used for all analysis except for BRCA1, ATM and the respective loading control where Tris-acetate gels were used to accommodate higher molecular weights of these proteins. The following antibodies were used: Actin (#4967), ATM (#2873), BRCA1 (#9010), gamma-H2AX (#80312), Rad51 (#8875), total-H2AX (#7631), p62 (#2947) and LC3BII (#2775) were from Cell Signaling (Danvers, MA, USA); CYP1B1 (ab185954) was from Abcam; β-tubulin (T7816) was from Sigma; DNA/RNA damage antibody (SMC-155) was from StressMarq (Victoria, BC, Canada) and Actin (#47778) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Bakewell S., Conde I., Fallah Y., McCoy M., Jin L, & Shajahan-Haq A.N. (2020). Inhibition of DNA Repair Pathways and Induction of ROS Are Potential Mechanisms of Action of the Small Molecule Inhibitor BOLD-100 in Breast Cancer. Cancers, 12(9), 2647.
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3

Western Blot Analysis of Cell Lysates

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For Western blot analysis, cells were lysed for 30 min at 4°C in lysis buffer (50 mM Tris-HCl, pH 7.5, containing 150 mM NaCl, 1 mM EDTA, 0.5% sodium deoxycholate, 1% IGEPAL CA-630, 0.1% sodium dodecyl sulfate (SDS), 1 mM Na3VO4, 44 µg ml−1 phenylmethylsulfonyl fluoride) supplemented with Complete Mini protease inhibitor mixture tablets (Roche Applied Science). Total protein was quantified using the bicinchoninic acid assay (Pierce). Whole-cell lysate (20 µg) was resolved by SDS–polyacrylaminde gel electrophoresis. The following antibodies were used: c-Myc (no. 5605), cyclinD1 (no. 2978), p21 (no. 2947) and RB1 (no. 9309) were from Cell Signaling, Danvers, MA; ESRα (MA5-14104) was from Invitrogen; RB1-phosphorylated on Ser612 (OAAB16108) was from Aviva Systems Biology, San Diego, CA; actin (sc-47778) was from Santa Cruz Biotechnology, Santa Cruz, CA; β-tubulin (T7816) was from Sigma, St. Louis, MO.
He W., Demas D.M., Conde I.P., Shajahan-Haq A.N, & Baumann W.T. (2020). Mathematical modelling of breast cancer cells in response to endocrine therapy and Cdk4/6 inhibition. Journal of the Royal Society Interface, 17(169), 20200339.
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