3-DSC (purity >98%) was purchased from AK Scientific, Inc (Union City, CA, USA). CellTiter-Glo®Luminescent Cell Viability Assay kit was purchased from Promega Corporation (Madison, WI, USA). DMEM and fetal bovine serum (FBS) were procured from Invitrogen (Carlsbad, CA, USA). Interleukin (IL)-6 and IL-4 were purchased from R&D systems (Minneapolis, MN, USA). β-actin antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). Polyclonal antibodies against total β-catenin, phospho-specific β-catenin (Thr41/Ser45), total STAT3, STAT6, phospho-specific STAT3 (Tyr705) and STAT6 (Tyr641) were purchased from Cell Signaling Technology (Beverly, MA, USA). All other chemicals used in our experiments were molecular biology grade.
Phospho specific stat3 tyr705
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-specific STAT3 (Tyr705) is a laboratory reagent used to detect and quantify the phosphorylation of STAT3 at tyrosine 705. It is a highly specific antibody that can be used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry, to analyze the activation and signaling of the STAT3 transcription factor.
Automatically generated - may contain errors
Lab products found in correlation
Stat6, by Cell Signaling Technology (1 mentions)
Stat6tyr641, by Cell Signaling Technology (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions)
Interleukin 4 (il 4), by R&D Systems (1 mentions)
Interleukin il 6, by R&D Systems (1 mentions)
Streptomycin, by Welgene (1 mentions)
Survivin, by Cell Signaling Technology (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Fibrillarin, by Cell Signaling Technology (1 mentions)
α tubulin, by Cell Signaling Technology (1 mentions)
Lps purified from escherichia coli o111 b4, by Merck Group (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Cleaved parp, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
Penicillin, by Welgene (1 mentions)
2 protocols using phospho specific stat3 tyr705
1
Cytotoxicity Evaluation of 3-DSC
2
Lung Cancer Cell Lines and Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The lung cancer cell lines H1299, A549, and H23 were purchased from the American Type Culture Collection (Manassas, VA, USA). All cell lines were cultured in RPMI1640 medium, 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% (v/v) fetal bovine serum (WelGENE, Daegu, Korea). Various antibodies against STAT3, phospho-specific STAT3 (Tyr 705), c-Myc, cyclin D1, survivin, PARP, cleaved PARP, α-tubulin, and fibrillarin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA) and EMD Millipore (Billerica, MA, USA) for use in western blotting. The ApopTag® Red In Situ Apoptosis Detection Kit (TUNEL) was purchased from EMD Millipore (Billerica, MA, USA). LPS purified from Escherichia coli O111:B4 was purchased from Sigma-Aldrich (St. Louis, MO, USA). The STX-0119 (Stat3 inhibitor) was synthesized by Dr. Dong Yun Shin, Gachon University, Republic of Korea.
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