Anti acetylated lysine lys ac antibody

For a custom anti-rabbit Hsp70-K77-Ac antibody, rabbits were immunized with synthetic peptide-bearing acetylated human Hsp70 (Ab_72–81:RLIGRK-acFGDP) following standard procedures (Peptron and Abfrontier). This antibody was purified with antigen-specific affinity chromatography and blocked by non-acetylated synthetic peptide (Ab_72–81:RLIGRKFGDP) before use. Anti-Hsp70 antibody (C92F3A-5, ADI-SPA-810, 1:3,000) and anti-Hsc70 antibody (1B5, ADI-SPA-815, 1:3,000) were purchased from Enzo Lifescience. Anti-Hop antibody (D6E3, #5669, 1:1,000), anti-Hsp40 antibody (#4868, 1:3,000), anti-acetylated lysine (Lys-Ac) antibody (#9941, 1:1,000), anti-cytochrome c antibody (#11940, 1:3,000), anti-cox-4 antibody (#4844, 1:3,000), anti-caspase-9 antibody (#9508, 1:3,000), anti-cleaved caspase-3 antibody (#9661, 1:3,000) and anti-HDAC4 antibody (#7628, 1:3,000) were from Cell Signaling Technology. Anti-Hsp90 antibody (H-114, sc-7947, 1:3,000), anti-CHIP antibody (H-231, sc-66830, 1:3,000), anti-Myc antibody (9E10, sc-40, 1:3,000), anti-ARD1 antibody (FL-235, sc-33820, 1:3,000) and anti-GST antibody (B-14, sc-138, 1:3,000) were purchased from Santa Cruz. The anti-FLAG antibody (M2, F1804, 1:3,000) and anti-tubulin antibody (DM1A, T9016, 1:3,000) were from Sigma. Anti-GFP antibody (ab6556, 1:3,000) and anti-ubiquitin antibody (Z0458, 1:3,000) were from Abcam and Dako, respectively.

Anti-acetylated lysine (Lys-Ac) antibody (#9941) were purchased from Cell Signaling Technology. Anti-his antibody (sc-8036), anti-GST antibody (sc-138) were from Santa Cruz.