To analyze swimming trajectory, opossum sperm cells were transferred to 37 °C H-HTF medium with 0.5% methylcellulose (w/v) in an imaging chamber coated with 0.2% agarose to minimize head attachment on the plate. Sperm movements were recorded for 2 s with 100 fps speed using Axio observer Z1 microscope with pco.edge sCMOS camera (Carl Zeiss, Oberkochen, Germany). Overlaid images to trace the sperm swimming paths were generated by using FIJI software.
Pco edge scmos camera
Manufactured by Zeiss
Sourced in Germany
The Pco.edge sCMOS camera is a high-performance scientific CMOS camera designed for demanding imaging applications. It features a large active sensor area, high resolution, and fast frame rates. The camera is capable of capturing high-quality images and videos with low noise and high dynamic range. The Pco.edge sCMOS camera is suitable for a variety of scientific and industrial applications that require advanced imaging capabilities.
Automatically generated - may contain errors
2 protocols using pco edge scmos camera
1
Opossum Sperm Swimming Trajectory
2
Yeast Mitochondrial Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The day before the experiment yeast were grown to saturation. On the day of experiment the saturated culture was diluted 1:50 in SD media without selection or with just auxotrophic selections. Cells were grown for 4 h and applied on glass-bottom plates coated with concanavalin A and left for 20 min to adhere. If mitochondria needed to be visualized, the solution was removed from the wells and 50 nM MitoTracker Orange CMTMRos (ThermoFisher #M7510) diluted in imaging media (SD media with complete set of amino acids but without riboflavin) was placed in the wells for 10 min. Imaging was performed in fresh imaging media. Cells were imaged using VisiScope Confocal Cell Explorer system consisting of Olympus IX83 microscope, Zeiss Yokogawa spinning disk scanning unit equipped with PCO-Edge sCMOS camera controlled by VisiView sofware. Images were recorded with 488 nm laser illumination for GFP channel and 561 nm laser illumination for MitoTracker Orange and 60 oil objective. Micrographs were cropped, and slightly adjusted for brightness and contrast using Fiji (Schindelin et al., 2012) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!