An enoxaparin reference standard was purchased from SANDOZ. 2-Aminoacridone (AMAC) and sodium cyanoborohydride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Three 13C-labeled 8-mer calibrants were synthesized using the chemoenzymatic synthesis method.10 (link) Recombinant heparan lyase I, II, and III were expressed in E. coli and purified by a Ni-agarose column. DEAE-Sepharose Fast Flow resin was purchased from GE Healthcare (Chicago, IL, USA). All reagents and chemicals were high-performance LC (HPLC) grade or LC-mass spectrum (MS) grade.
2 aminoacridone
Manufactured by Merck Group
Sourced in United States
2-Aminoacridone is a fluorescent dye used as a labeling agent in various analytical techniques, such as high-performance liquid chromatography (HPLC) and capillary electrophoresis. It is a chemical compound with the molecular formula C₁₃H₁₀N₂O.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 2 aminoacridone
1
Chemoenzymatic Synthesis of 13C-labeled Heparan Sulfate
2
Disaccharide Standards for Glycosaminoglycan Analysis
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Unsaturated disaccharide standards of chondroitin sulphate (DUA-GalNAc; DUA-GalNAc4S; DUA-GalNAc6S; DUA2S-GalNAc; DUA2S-Gal-NAc4S; DUA2S-GalNAc6S; DUAGalNAc4S6S; DUA2S-GalNAc4S6S), unsaturated disaccharide standards of Heparan sulpahte (DUA-GlcNAc; DUA-GlcNS; DUA-GlcNAc6S; DUA2S-GlcNAc; DUA2S-GlcNS; DUAGlcNS6S; DUA2S-GlcNAc6S; DUA2S-GlcNS6S), and unsaturated disaccharide standard of hyaluronan (DUA-GlcNAc), where DUA is 4-deoxy-α-L-threo-hex-4enopyranosyluronic acid, were purchased from Iduron (UK). Recombinant Flavobacterial heparin lyases I, II, and III were expressed using Escherichia coli strains in our Laboratory.
2-Aminoacridone and sodium cyanoborohydride were obtained from Sigma-Aldrich (St.
Louis, MO, USA). All other chemicals were of HPLC grade.
2-Aminoacridone and sodium cyanoborohydride were obtained from Sigma-Aldrich (St.
Louis, MO, USA). All other chemicals were of HPLC grade.
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