Ke1002

Manufactured by Proteintech

KE1002 is a high-performance electrophoresis power supply designed for a variety of laboratory applications. It provides a stable and adjustable output voltage and current for running gel electrophoresis experiments.

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Lab products found in correlation

Spectrafluor plus, by Tecan (3 mentions) Ke1003, by Proteintech (2 mentions)

3 protocols using ke1002

Cytokine Quantification in Chondrocytes

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The concentrations of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and interleukin-10 (IL-10) were determined in the culture supernatants from chondrocytes treated with different stimuli using commercial ELISA Kits (cat# KE1002, KE1003, Proteintech) following the manufacturer’s instructions. The absorbance at 450 nm was measured using a Multiskan Ascant (SPECTRAFluor Plus, Tecan).

Cui Y., Song H.T., Zhang P., Yin X., Wang Y., Wei X, & Jia X.J. (2022). Curcumin protects PC12 cells from a high glucose-induced inflammatory response by regulating the miR-218-5p/TLR4 axis. Medicine, 101(40), e30967.

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Quantifying Inflammatory Cytokines in Chondrocytes

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The concentrations of IL-1β and IL-6 in the culture supernatants from chondrocytes treated with the different stimuli were determined using commercial ELISA kits (cat. nos. KE1002 and KE1003; Proteintech Group, Inc.) following the manufacturer's instructions. The absorbance at 450 nm was detected with a Multiskan Ascant (SPECTRAFluor Plus; Tecan Group, Ltd.).

Qi H.L., Chen Z.Y., Qin Y.H, & Li Y.Z. (2022). Curcumin ameliorates H2O2-induced inflammatory response in chondrocytes by inducing autophagy activation. Experimental and Therapeutic Medicine, 23(4), 272.

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ELISA Quantification of Cytokines

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The concentrations of IL-1β, IL-6, and TNF-α were determined in the culture supernatants from 1 × 10⁷ cells/well VSMCs treated with the different stimuli using a commercial ELISA kit (Proteintech, KE1002, KE1003, KE1007) according to the manufacturer’s instructions. The absorbance at 450 nm was detected with a Multiskan Ascent (SPECTRAFluor Plus, Tecan).

Qin Y., Zheng B., Yang G.S., Yang H.J., Zhou J., Yang Z., Zhang X.H., Zhao H.Y., Shi J.H, & Wen J.K. (2020). Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells. Molecular Therapy. Nucleic Acids, 21, 492-511.

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