Trypsin with edta

Human umbilical vascular endothelial cells (HUVECs), trypsin with EDTA, trypsin neutralizing solution, endothelial cell growth medium-2 (EGM-2) with hydrocortisone, hFGF-B, vascular endothelial growth factor (VEGF), R3-IGF-1, ascorbic acid, hEGF, GA-1000, heparin and fetal bovine serum (FBS), were purchased in Lonza (Basel, Switzerland). Endonuclease III (Nth) and human 8-oxoguanine DNA glycosylase (hOGG1) were acquired in New England Biolabs (Ipswich, MA, USA). 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA) was purchased from Invitrogen (Waltham, Massachusetts, USA). Low melting point (LMP), normal melting point (NMP) agarose, 4′,6-diamidino-2-phenylindole (DAPI), dabigatran, rivaroxaban and 25-hydroxycholesterol (25-OHC) were bought from Sigma-Aldrich (St. Louis, MO, USA) while other chemicals were purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany) and POCh (Gliwice, Poland) and were of analytical grade.

Trypsin with EDTA, trypsin neutralizing solution, endothelial cell growth medium-2 (EGM-2) with hydrocortisone, hFGF-B, VEGF, R3-IGF-1, ascorbic acid, hEGF, GA-1000, heparin, and fetal bovine serum (FBS), were purchased in Lonza (Basel, Switzerland). Empagliflozin (11575), dapagliflozin (11575), and canagliflozin (11575) were purchased from Cayman Chemical (Ann Arbor, Michigan, USA) and 25-OHC was purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibodies used for immunofluorescence: VE-Cadherin (D87F2) XP^® Rabbit mAb #2500 were purchased from Cell Signaling (Warszawa, Poland), and anti-Rabbit (H+L) Alexa Fluor Plus 488 was purchased from Invitrogen (Waltham, MA, USA).