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Total glp 1 ver 2 assay kit

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The Total GLP-1 (ver. 2) Assay Kit is a laboratory equipment product designed to measure the total level of Glucagon-Like Peptide-1 (GLP-1) in various sample types. The kit utilizes an enzyme-linked immunosorbent assay (ELISA) technology to quantify the total GLP-1 concentration.

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Lab products found in correlation

Human gip total or human ghrelin total elisa kit, by Merck Group (2 mentions) Nefa hr 2, by Fujifilm (1 mentions) Matrigel, by BD (1 mentions) Fatty acid free bsa, by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions) Olive oil, by Merck Group (1 mentions)

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Total GLP-1 assay (9 citations) Total GLP-1 assay kit (2 citations) Total GLP-1 (2 citations) GLP-1 (2 citations)

4 protocols using total glp 1 ver 2 assay kit

1

Comprehensive Metabolic Analysis Protocol

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Plasma glucose was analyzed by an ABL800 FLEX blood gas analyzer, and serum insulin and C-peptide were analyzed on a Cobas e 411 with an electrochemiluminescence immunoassay (ECLIA). Insulin intra- and inter-assays had a CV% of 1.9–2.0 and 2.5–2.6, respectively. The C-peptide intra- and inter-assay CV% were 1.3–4.6 and 1.8–5.0, respectively. Plasma free fatty acids (FFA) were analyzed using the in vitro enzymatic colorimetric method assay NEFA-HR(2) (Wako Chemicals, GmbH, Neuss, Germany). Plasma GLP-1 was assessed by the Total GLP-1 (ver. 2) Assay Kit from Meso Scale Discovery. Plasma GIP and ghrelin were assessed by using the Human GIP (Total) or Human Ghrelin (Total) ELISA Kit from Millipore. Intra- and inter-assay CV% of the GIP kit was 3.0–8.8 and 1.8–6.1, and the intra- and inter-assay CV% of the ghrelin kit was 0.90–1.91 and 5.18–7.81.
Sørensen K.V., Kaspersen M.H., Ekberg J.H., Bauer-Brandl A., Ulven T, & Højlund K. (2021). Effects of Delayed-Release Olive Oil and Hydrolyzed Pine Nut Oil on Glucose Tolerance, Incretin Secretion and Appetite in Humans. Nutrients, 13(10), 3407.
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2

Colonic Crypt GLP-1 Secretion Assay

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Colonic crypts were prepared from male C57BL/6 WT, Gpr40 KO or Gpr40/120 double KO littermate mice (obtained from Taconics, NY). The crypts were isolated by collagenase digestion as described by Reimann et al. [23] (link) and seeded into 48-well plates coated with Matrigel (BD Biosciences). The following day, cells were incubated for 2 h with ligands (triplicates) at 37 °C in standard solution [23] (link) containing 0.1% fatty acid-free BSA (Sigma–Aldrich) and 10 mM glucose. Synthetic ligands were dissolved in DMSO and ALA or DHA were complexed with free fatty acid BSA in a 6:1 molar ratio. Total GLP-1 was determined using Total GLP-1 (ver. 2) assay kit (Meso Scale Discovery, Gaithersburg, USA) (model number K150JVC-1) according to manufacturer's protocol.
Hauge M., Vestmar M.A., Husted A.S., Ekberg J.P., Wright M.J., Di Salvo J., Weinglass A.B., Engelstoft M.S., Madsen A.N., Lückmann M., Miller M.W., Trujillo M.E., Frimurer T.M., Holst B., Howard A.D, & Schwartz T.W. (2014). GPR40 (FFAR1) – Combined Gs and Gq signaling in vitro is associated with robust incretin secretagogue action ex vivo and in vivo. Molecular Metabolism, 4(1), 3-14.
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3

Biomarker Measurements for Metabolic Health

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Blood glucose was measured using the ABL800 FLEX blood gas analyzer. Insulin and C-peptide were analyzed using an electrochemiluminescence immunoassay (ECLIA) on Cobas e 411. The insulin intra-assay CV% was 1.9-2.0 and the inter-assay CV% was 2.5-2.6. For C-peptide the intra-assay CV% was 1.3-4.6 and the inter-assay CV% was 1.8-5.0. Moreover, we determined GLP-1 levels using Total GLP-1 (ver. 2) Assay Kit (Meso Scale Discovery). GIP and ghrelin were measured using the corresponding Human GIP (Total) or Human Ghrelin (Total) ELISA Kit (Millipore). The GIP kit had an intra-assay CV% of 3.0-8.8 and an inter-assay CV% of 1.8-6.1 and the ghrelin kit had an intra-assay CV% of 0.90-1.91 and an inter-assay CV% of 5.18-7.81.
Sørensen K.V., Korfitzen S.S., Kaspersen M.H., Ulven E.R., Ekberg J.H., Bauer-Brandl A., Ulven T, & Højlund K. (2021). Acute effects of delayed-release hydrolyzed pine nut oil on glucose tolerance, incretins, ghrelin and appetite in healthy humans. Clinical nutrition (Edinburgh, Scotland), 40(4).
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4

Olive Oil Modulates GLP-1 and GIP Levels

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Mice (9-29 weeks) were fasted overnight (16-18 hours) with ad libitum access to water. Olive oil (Sigma Aldrich) was administered p.o. with a dose of 10 ml/kg. A retro orbital blood sample (100 µl) was taken prior to the dosage and was considered 0 min. 60 minutes after the dosage, animals were decapitated and blood was collected. All blood samples were collected in EDTA coated tubes with 0.5 KIU aprotinin/µl blood (Sigma Chemical, St. Louis, MO) and 1 µM DPP4 inhibitor. All blood samples were kept on ice at all times. The blood samples were spun at 9600 G for 10 minutes at 4°C and plasma was stored at -80°C. Total GLP-1 was determined using Total GLP-1 (ver. 2) assay kit (Meso Scale Discovery, Gaithersburg, USA) (model number K150JVC-1). GIP was measured using a Rat/Mouse Total GIP ELISA assay kit (Millipore, St. Charles, MO).
Ekberg J.P., Hauge M., Kristensen L.V., Madsen A.N., Engelstoft M.S., Husted A.S., Sichlau R., Egerod K., Kowalski T., Gribble F.M., Reimann F., Hansen H.S., Howard A.D., Holst B, & Schwartz T.W. (2016). GPR119 - a major Enteroendocrine Sensor of Dietary Triglyceride Metabolites Co-acting in Synergy with FFA1 (GPR40). Endocrinology, 157(12), 4561-4569.
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