Rabbit anti acetylated histone h3

Western blot analysis was performed as described previously using the following antibodies: mouse anti-phosphorylated IκBα (Cell Signaling, Danvers, MA, USA), rabbit anti-IκBα (Cell Signaling), rabbit anti-acetylated histone H3 (Millipore, Billerica, MA, USA), rabbit anti-NIK (Cell Signaling), mouse anti-p100/p52 (Millipore), rabbit anti-phosphorylated p65 (Cell Signaling) and rabbit anti-p65 (Abcam, Cambridge, MA, USA). Mouse anti-α-Tubulin (Sigma) and mouse anti-GAPDH (HyTest, Turku, Finland) were used as loading controls. Goat anti-mouse IgG and goat anti-rabbit IgG conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Dallas, TX, USA) as secondary antibodies and enhanced chemiluminescence were used for detection (Amersham Bioscience, Freiburg, Germany). Alternatively, infrared dye-labeled secondary antibodies and infrared imaging were used for detection (Odyssey imaging system, LI-COR Bioscience, Bad Homburg, Germany). Representative blots of at least two independent experiments are shown.

Antibodies used for western blot analysis were rabbit anti-CSB (1:2000) (21 (link)), rabbit anti-CTCF (1:2000) (Millipore, 07-729), mouse anti-GAPDH (1:10 000) (Millipore, MAB374), rabbit anti-BRG1 (1:1000) (22 (link)), rabbit anti-acetylated histone H3 (1:1000) (Millipore, 06-599), HRP-conjugated goat anti-rabbit IgG (1:10 000) (Pierce, 31460) and HRP-conjugated goat anti-mouse (IgG+IgM) (1:10 000) (Jackson Laboratory, 115-035-044).