Cytotoxicity detection kitplus ldh assay

PHFK and N/TERT-2G were plated at a density of 75,000 cells/well in 96-well plates. Cells were treated with S. aureus conditioned media or purified SEl toxins at the time of differentiation. Cell culture media and a lysed cell control were collected 48 h later for use in the Cytotoxicity Detection Kit^PLUS (LDH assay - Roche). The LDH assay was incubated for 10 min and then read at 490 nm/620 nm wavelength. A 1:10 dilution of WST-1 (Cell Proliferation Reagent WST-1, Roche) was added to the cell culture wells (final dilution of 1:20 for WST-1 reagent) 48 h after treatment and incubated at 37°C for 2 h after the addition of the reagent. The WST-1 assay was read at 420 nm/620 nm.

PHFK and N/TERT-2G were plated at a density of 75,000 cells/well in 96-well plates. Cells were treated with S. aureus conditioned media or purified SEl toxins at the time of differentiation. Cell culture media and a lysed cell control were collected 48 h later for use in the Cytotoxicity Detection Kit^PLUS (LDH assay - Roche). The LDH assay was incubated for 10 min and then read at 490 nm/620 nm wavelength. A 1:10 dilution of WST-1 (Cell Proliferation Reagent WST-1, Roche) was added to the cell culture wells (final dilution of 1:20 for WST-1 reagent) 48 h after treatment and incubated at 37°C for 2 h after the addition of the reagent. The WST-1 assay was read at 420 nm/620 nm.