Dylight 775 b4

Isopropyl-β-thiogalactopyranoside (IPTG) and carbenicillin were purchased from Fisher Scientific (Pittsburg, PA). Traut's reagent (2-iminothiolane), heparin sulfate, MES (2-(N-morpholino) ethanesulfonic acid), EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide Hydrochloride) and TRITC (rhodamine B isothiocyanate) were purchased from Sigma-Aldrich (St. Louis, MO). BL21star (DE3) E. coli strain, AcTEV™ protease, Hybridoma serum free medium (SFM), Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum albumin (FBS) and PBS (pH 7.4) were purchased from Invitrogen (Carlsbad, CA). Dylight 488 and Dylight 775-B4 were purchased from Thermo Scientific (Rockford, IL). PEG (NH₂-PEG-MAL; 3.5 kDa) was purchased from JenKem Technology USA Inc. (Allen, TX).

Carbenicillin and isopropyl-β-thiogalactopyranoside (IPTG) were purchased from Fisher Scientific (Pittsburg, PA). Heparin sulfate and rhodamine B isothiocyanate (TRITC), Traut’s reagent (2-iminothiolane), MES (2-(N-morpholino) ethanesulfonic acid), EDC (1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide Hydrochloride), goat anti-mouse-IgG (Fc specific)-alkaline phosphatase, rat plasma and nonspecific murine IgG were purchased from Sigma-Aldrich (St. Louis, MO). Competent Escherichia Coli (E. coli) strains (BL21star (DE3)), AcTEV™ protease, PBS (pH 7.4), Dulbecco’s Modified Eagle Medium (DMEM), Hybridoma serum free medium (SFM), fetal bovine serum albumin (FBS) were purchased from Invitrogen (Carlsbad, CA). One-step PNPP, Dylight 679-C5 and Dylight 775-B4 were purchased from Thermo Scientific (Rockford, IL). Polyethylene glycol (NH₂-PEG-MAL; 3.5 kDa) was purchased from JenKem Technology USA Inc. (Allen, TX). Recombinant CEA (rCEA) was purchased from R&D SYSTEMS (Minneapolis, MN). Restriction endonucleases (BamHI & XhoI) were purchased from New England Biolabs (Ipswich, MA). The pET28a-Gel vector for the expression of recombinant gelonin was a kindly gift from Dr. Wolfgang. E. Trommer (TU Kaiserslautern, Germany) [24 (link)].