CCL11 content of the serum of patients with BC and healthy individuals was measured by ELISA, according to the manufacturer's protocol. The Human Eotaxin ELISA kit (cat. no. ab185985; Abcam) was used to measure CCL11 levels, according to the manufacturer's protocol. The IL-2 and TGF-β1 content of the media of CD4+ T cells from the PBMCs of patients with BC or healthy individuals was measured using the Human IL-2 Quantikine ELISA kit (cat. no. S2050; R&D Systems) and the Human TGF-β1 Quantikine ELISA kit (cat. no. SB100B; R&D Systems), respectively. The CCL11, IL-2 and TGF-β1 content of the serum of tumor-bearing mice was measured using the Mouse CCL11/Eotaxin Quantikine ELISA kit (cat. no. MME00; R&D Systems), the Mouse IL-2 Quantikine ELISA kit (cat. no. SM2000; R&D Systems) and the Mouse/Rat/Porcine/Canine TGF-β1 Quantikine ELISA kit (cat. no. SMB100B; R&D Systems), respectively.
Mouse rat porcine canine tgf β1 quantikine elisa kit
Manufactured by R&D Systems
The Mouse/Rat/Porcine/Canine TGF-β1 Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure TGF-β1 levels in cell culture supernates, serum, plasma, and urine samples.
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3 protocols using mouse rat porcine canine tgf β1 quantikine elisa kit
1
Quantifying Immune Markers in Breast Cancer
2
TGFβ Quantification in Keratinocyte Media
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200,000 mouse normal keratinocytes and A223, LY2, A1419 cells were plated on a 6-well plate for 24 h to adhere. Media was replaced with 1.5 mL of DMEM/F12 supplemented with 0.5% FBS, which was collected after 24 h for analysis. Media samples were then analyzed for TGFβ levels using the Mouse/Rat/Porcine/Canine TGF-β1 Quantikine ELISA kit (R&D Systems) according to the manufacturer’s instructions, corrected for baseline TGFβ levels from the media alone.
3
TGF-β1 Quantification from Mouse and Dog Skin
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