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Nano series zetasizer nano zs90

Manufactured by Malvern Panalytical
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The Nano Series Zetasizer Nano ZS90 is a dynamic light scattering (DLS) instrument used for the measurement of particle size and molecular weight. It is capable of analyzing samples with particle sizes ranging from 0.3 nanometers to 10 micrometers. The Zetasizer Nano ZS90 utilizes a laser and a detector to measure the Brownian motion of particles in a sample, which is then used to determine their size distribution.

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3 protocols using nano series zetasizer nano zs90

1

Zeta-Potential Analysis of Emulsions

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Measurement of the ζ-potential of emulsions was determined by a Zetasizer Nano Series (Zetasizer Nano-ZS90, Malvern Instruments, Worcestershire, UK). After diluting the samples with MilliQ water, the emulsions’ pH (3.0, 6.0, and 9.0) was set using HCl and NaOH. Then, the samples were filled into capillary cells to measure zeta potential. The samples were diluted with deionized water and filled into the test cell before measurement. This experiment was performed to check the stability of emulsions on different pH as well. Each sample took three readings with triplicate measurements.
Muhammad Z., Ramzan R., Zhang R., Zhao D., Khalid N., Deng M., Dong L., Aziz M., Batool R, & Zhang M. (2022). Enhanced Bioaccessibility of Microencapsulated Puerarin Delivered by Pickering Emulsions Stabilized with OSA-Modified Hydrolyzed Pueraria montana Starch: In Vitro Release, Storage Stability, and Physicochemical Properties. Foods, 11(22), 3591.
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2

Nanoparticle Size Characterization using Zetasizer

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sEV size was determined using Malvern Nano Series Zetasizer Nano ZS90. A sEV aliquot of 50 µl was added to 450 µl nuclease-free water and mixed in a cuvette for determining the size of sEV in the instrument.
Kong X., Patel N.A., Chalfant C.E, & Cooper D.R. (2023). Ceramide synthesis regulates biogenesis and packaging of exosomal MALAT1 from adipose derived stem cells, increases dermal fibroblast migration and mitochondrial function. Cell Communication and Signaling : CCS, 21, 221.
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3

Nanoparticle Size Determination via Dynamic Light Scattering

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SEV size was determined using Malvern Nano Series Zetasizer Nano ZS90. A SEV aliquot of 50 µl was added to 450 µl nuclease-free water and mixed in a cuvette for determining the size of SEV in the instrument.
Kong X., Patel N.A., Chalfant C., & Cooper D.R. (2021). Ceramide synthesis regulates biogenesis and packaging of exosomal MALAT1 from adipose derived stem cells, increases dermal fibroblast migration and mitochondrial function.
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