Cd8 pe 53 6.7

Manufactured by BioLegend

CD8± PE (53-6.7) is a fluorochrome-conjugated antibody that binds to the CD8 antigen expressed on the surface of cytotoxic T cells. This product is intended for use in flow cytometry applications to identify and quantify CD8+ T cell populations.

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CD8-PE (65 citations) CD8 (53-6.7, (38 citations) Anti-CD8-PE-Cy7 (53-6.7), (4 citations) Anti-CD8-PE/Cy5 (clone 53–6.7; (3 citations) Anti-CD8 (53-6.7) PE-Cy (2 citations) Anti-mouse CD8-PE (clone 53-6,7, (2 citations) Anti-Mouse-CD8-PE/Cy7 (53-6.7) (2 citations)

2 protocols using cd8 pe 53 6.7

Multiparametric Analysis of Immune Cell Subsets

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Antibodies were from the following sources: CD4 Alexa 488 or Alexa 405 (GK1.5), TS1 Alexa 647 (6.5) and F4/80 Alexa 488 (HB-198) were lab-prepared. CD25 (R-phycoerythrin (PE) (7D4)) was from Southern Biotech. FOXP3 (150D), CD62L PE-Cy7 (Mel-14), PD-1 PE (29F.1A12), CD8± PE (53-6.7) and CD44 APC-Cy7 (1M7) were from BioLegend. Gr-1 PE (RB6-8C5) and CD11b PE (MI/70) were from eBioscience. CD4 PE (RM4-5; BD) and CD8 PE (YTSI56.7.7; Biolegend), which could bind in the presence of the depleting antibodies, were used for confirmation of in vivo T cell depletions. Ethidium monoazide (EMA, Invitrogen) or propidium iodide were used to exclude dead cells.

Juchem K.W., Sacirbegovic F., Zhang C., Sharpe A.H., Russell K., McNiff J.M., Demetris A.J., Shlomchik M.J, & Shlomchik W.D. (2017). PD-L1 Prevents the Development of Autoimmune Heart Disease in Graft-vs-Host Disease. Journal of immunology (Baltimore, Md. : 1950), 200(2), 834-846.

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Murine Immune Response Analysis

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Lymph nodes and spleen tissues were collected after mice were euthanatized on day 30. Single cells were obtained by grinding tissues with 70 μm nylon filters in 0.5% BSA-PBS. Anti-mouse antibodies against CD3-Percp/Cy5.5 (145-2C11, BioLegend), CD4-APC (GK1.5, BioLegend), CD4-FITC (GK1.5, BioLegend), CD8-PE (53-6.7, BioLegend), CD11c-FITC (N418, BioLegend), CD11b-Percp/Cy5.5 (M1/70, BioLegend), CD80-PE (16-10A1, eBioscience), CD86-APC (24F, BioLegend), CD69-FITC (H1.2F3, BioLegend), B220-Percp/Cy5.5 (RA3-6B2, BioLegend), CD138-APC (281-2, BioLegend) or I-A/I-E (MHC II)-PE/Cy7 (M5/114.15.2, BioLegend) were used for cell surface antigen staining. After washing with PBS for three times, cells were treated with Foxp3/transcription factor fixation/permeabilization concentrate and diluent (eBioscience, USA) and incubated with anti-mouse antibodies against IFNγ-PE/Cy7 (XMG1.2, BioLegend) or Ki67-FITC (SolA15, BioLegend) for intracellular antigen staining. Besides, S1 protein with His tag (Sino Biological, China) and anti-His tag-PE (BioLegend) were used to label S1 specific B cells. The cells were analyzed by flow cytometry.

Zhang M., Wang L., Liu J, & Pang Y. (2022). Envelope virus-mimetic nanovaccines by hybridizing bioengineered cell membranes with bacterial vesicles. iScience, 25(6), 104490.

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