Cd34 sc 74499

Antibodies against VEGF (SC-507), focal adhesion kinase (FAK; SC-932), Src (SC-5226), Akt (SC-5298), and CD34(SC-74499) were bought from Santa Cruz (Santa Cruz, CA, USA). Antibodies targeting p-FAK (3283S), p-Src (2101S), p-AKT (4060S), and CD133(64326s) were obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA). Antibodies against CD31(ab28364) was purchased from Abcam (Cambridge, MA, USA). Small interfering RNAs (siRNAs) against FAK (L-003164-00), Src (L-003110-00) and Akt (L-003000-00-0005) and their respective controls were purchased from Dharmacon (Lafayette, CO, USA). We purchased VEGF shRNA plasmids from the National RNAi Core (Taipei, Taiwan). Inhibitors for FAK (869288-64-2) were from Calbiochem (San Diego, CA, USA). A VEGF ELISA kit (DY293B) was purchased from R&D Systems (Minneapolis, MN, USA) and APLN ELISA (KA1681) kit was purchased from Abnova (Taipei, Taiwan). Inhibitors for Src (P0042), Akt (A6730) and all the chemicals not mentioned above were supplied by Sigma-Aldrich (St. Louis, MO, USA).

Recombinant human VEGF (100-20) was bought from PeproTech (Rocky Hill, NJ, USA). VEGFR2 (07-158), phospho-VEGFR2 (orb106137), CD133 (orb13002), and β-actin (a5441) antibodies, and melatonin (M5250) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phospho-FAK (3283S) antibodies was purchased from Cell Signaling (Danvers, MA, USA). FAK (sc-1688), phospho-c-Src (sc-12928-R), c-Src (2105S), phospho-c-Jun (sc-822), c-Jun (sc-74543), phospho-p65 (sc-101752), p65 (sc-8008), CD31 (sc-18916), and CD34 (sc-74499) antibodies, as well as the angiotensin II (FAK activator; sc-363643) and c-Src activator (sc-3052), were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The NF-κB activator (prostratin; ab120880) was purchased from Abcam (Cambridge, MA, USA). PDGF-BB antibody (MBS9404630) was purchased from MyBioSource (San Diego, CA, USA). The VEGFR2 short hairpin RNA (shRNA) plasmid was purchased from the National RNAi Core Facility Platform (Taipei, Taiwan).