The FA oxidation was monitored using HPLC (Shimadzu Class-VP HPLC, Tokyo, Japan). Separation of products was performed by LiChro-CART RP-18 column (Merck, 25 × 0.4 × 5 µm). The products were studied using a photodiode-array detector (PDA-M10A VP) at 20 °C without heating the column. Two solvents were used to realize the elution. Solvent A was composed from water and TFA (0.03; v/v), and solvent B was composed from 80% acetonitrile and 20% solvent A, with a flow rate of 0.7 mL/min. The gradient was used as follows: linear from 5% to 30% of solvent B for 14 min, from 30% to 55% of solvent B for 10 min and from 55% to 75% of solvent B for 10 min. Each analysis was made in triplicate.
Class vp hplc
Manufactured by Shimadzu
Sourced in Japan
The Class VP HPLC is a high-performance liquid chromatography system designed for laboratory use. It performs the core function of separating and analyzing complex mixtures of chemical compounds.
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Lab products found in correlation
3 protocols using class vp hplc
1
HPLC Analysis of FA Oxidation
2
HPLC Analysis of Biogenic Amines
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Biogenic amines (histamine, tyramine, tryptamine and 2-phenylethylamine) and their precursors were detected following the procedure previously reported (Bordiga et al., 2020) . The chromatographic system used was a Shimadzu Class VP HPLC equipped with a UV-Vis detector SPD-10A. The column used was a Spherisorb S5 ODS2 (250 mm 9 4.6 mm I.D., particle size 5 lm). Eluent A: aqueous solution (adjusted pH to 3.5 with phosphoric acid) with heptane sulphonate (8.3 mM), KH 2 PO 4 (9.0 mM) and 20 lL L À1 of octylamine. Eluent B: Methanol.
3
HPLC Analysis of Biogenic Amines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biogenic amines (histamine, tyramine, tryptamine and 2-phenylethylamine) and their precursors were detected following the procedure previously reported (Bordiga et al., 2020) . The chromatographic system used was a Shimadzu Class VP HPLC equipped with a UV-Vis detector SPD-10A. The column used was a Spherisorb S5 ODS2 (250 mm 9 4.6 mm I.D., particle size 5 lm). Eluent A: aqueous solution (adjusted pH to 3.5 with phosphoric acid) with heptane sulphonate (8.3 mM), KH 2 PO 4 (9.0 mM) and 20 lL L À1 of octylamine. Eluent B: Methanol.
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