Escherichia coli cicc10899

The LAB strains of Lactobacillus rhamnosus grx19, L. acidophilus ss, L. plantarum s7, L. casei g9, Bifidobacterium animalis BLC and Streptococcus thermophiles 90–57 were isolated from the gut of subjects from Bama longevity, Guangxi province, China, in 2013. The strains were separately cultured in skim milk broth for 12 h at 37 °C. The bacteria and supernatant were separated by centrifugation. The harvested bacteria were suspended by saline to 1 × 10⁸ CFU mL⁻¹. The supernatant was filtered by 0.22 μm Millipore filtration for the assay.
Escherichia coli CICC10899, Salmonella enterica WX29, Staphylococcus aureus CICC10201, Pseudomonas brenneri CICC10271, Clostridium difficile CICC22951, Bacillus subtilis CICC10012 were bought from China Center of Industrial Culture Collection (Beijing, China). All the strains were cultivated in Luria–Bertani broth at 37 °C except the Clostridium difficile CICC22951 cultivated in Reinforced Clostridium Medium broth. The bacteria were harvested after incubation for12 h and suspended to 1 × 10⁷ CFU mL⁻¹ by saline.