Inverted confocal sp5

Manufactured by Leica

The Leica Inverted Confocal SP5 is a high-performance microscope system designed for advanced confocal imaging. It features a modular design and offers a range of capabilities for visualizing and analyzing biological samples. The system's core function is to provide high-resolution, three-dimensional imaging of specimens using confocal laser scanning technology.

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Lab products found in correlation

Metamorph software, by Leica (2 mentions) Nanozoomer 2.0 ht scanner, by Hamamatsu Photonics (2 mentions) Ndp view2 viewing software, by Hamamatsu Photonics (1 mentions) Bright field microscope, by Leica (1 mentions) Axioscan, by Zeiss (1 mentions) Las af software, by Leica (1 mentions) Zen software, by Zeiss (1 mentions) Ndp view2, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

SP5 DMI inverted confocal microscope SP5 inverted confocal laser microscope Leica TCS SP5 DMI6000 CS inverted confocal system SP5 inverted confocal laser scanning microscope SP5 Inverted 2-Photon FLIM confocal Inverted SP5 confocal microscopy TCS SP5 AOBS inverted confocal SP5 inverted confocal scanning microscope Leica SP5 X MP Inverted Laser Scanning Confocal Microscope SP5 Inverted Confocal Microscope (DMI6000CS)

2 protocols using inverted confocal sp5

Quantifying Pericyte and Fibroblast Populations

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Histological slides were scanned using Nanozoomer 2.0 HT scanner with a 40× objective and visualized with NDP.view2 viewing software (Hamamatsu). Fluorescent images were acquired on a brightfield microscope (Leica) using Metamorph software, inverted Confocal SP5 (Leica) using the Leica LAS AF software, or an Andor Dragonfly Spinning Disk Confocal microscope employing Imaris software. Quantification of PC and vimentin^positive fibroblasts was done on at least five random fields (about 1 mm² each) per sample using Imaris and ImageJ software. Images were processed with ImageJ. Images were assembled and adjusted with Adobe Photoshop/ Illustrator. Counting of PC was performed using z‐stack acquisition. Co‐staining with cell markers was confirmed on distinct layers. In analyses of human biopsies, PC numbers were calculated either per mm² or per Vimentin‐stained area using an arbitrary unit (AU corresponding to 10,000 square pixel) of vimentin^positive cells.

Paul C., Tang R., Longobardi C., Lattanzio R., Eguether T., Turali H., Bremond J., Maurizy C., Gabola M., Poupeau S., Turtoi A., Denicolai E., Cufaro M.C., Svrcek M., Seksik P., Castronovo V., Delvenne P., de Laurenzi V., Da Costa Q., Bertucci F., Lemmers B., Pieragostino D., Mamessier E., Janke C., Pinet V, & Hahne M. (2022). Loss of primary cilia promotes inflammation and carcinogenesis. EMBO Reports, 23(12), e55687.

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Multimodal Imaging of Biological Samples

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Histological slides were scanned using the Nanozoomer 2.0 HT scanner with a ×40 objective, and visualized with the NDP.view2 software (Hamamatsu). Fluorescent images were acquired with Axioscan using ZEN software (Zeiss), brightfield Axioimager Z1 (Zeiss) using the Metamorph software or on the inverted Confocal SP5 (Leica) using the Leica LAS AF software. Images were processed with Adobe Photoshop CS6.

Maurizy C., Abeza C., Lemmers B., Gabola M., Longobardi C., Pinet V., Ferrand M., Paul C., Bremond J., Langa F., Gerbe F., Jay P., Verheggen C., Tinari N., Helmlinger D., Lattanzio R., Bertrand E., Hahne M, & Pradet-Balade B. (2021). The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium. Nature Communications, 12, 4810.

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