Nf κb jurkat gfp transcriptional reporter cells

Manufactured by System Biosciences

Sourced in United States

NF-κB/Jurkat/GFP Transcriptional Reporter cells are a cell line engineered to express Green Fluorescent Protein (GFP) under the control of the NF-κB transcriptional response element. This cell line can be used to monitor NF-κB pathway activation in a quantitative manner.

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Lab products found in correlation

Hek293t, by Thermo Fisher Scientific (1 mentions) Jurkat, by American Type Culture Collection (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Endothelial cell growth medium 2 egm 2, by Lonza (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Expi293f cells, by Thermo Fisher Scientific (1 mentions) Expi293 expression medium, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

NF-κB (2 citations) GFP reporter (2 citations) NF-κB/Jurkat/GFPTM Transcriptional Reporter cell line (2 citations)

2 protocols using nf κb jurkat gfp transcriptional reporter cells

Culturing HEK293T and Jurkat Cell Lines

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We cultured HEK293T (Thermo Scientific) in DMEM supplemented with 10% FBS, 100 U/ml penicillin G and 100 μg/ml streptomycin at 37 °C in a humidified atmosphere under 5% CO2. We grew Jurkat (ATCC) and the NF-κB/Jurkat/GFP Transcriptional Reporter cells (System Biosciences) and the CTCL cell lines HH, HUT78, HUT102, SeAX (ATCC under similar conditions in RPMI 1640 supplemented with 10% FBS and 100 U/ml penicillin G and 100 μg/ml streptomycin. Cell lines were regularly tested for mycoplasma contamination.

da Silva Almeida A.C., Abate F., Khiabanian H., Martinez-Escala E., Guitart J., Tensen C.P., Vermeer M.H., Rabadan R., Ferrando A, & Palomero T. (2015). The mutational landscape of cutaneous T-cell lymphoma and Sézary syndrome. Nature genetics, 47(12), 1465-1470.

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Cell Culture Conditions for SCLC and HUVEC

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NCI-H69, NCI-H146, and NCI-H187 human SCLC cells (Korean Cell Bank, Seoul, Korea), and NF-κB/Jurkat/GFP™ transcriptional reporter cells (System Bioscience, Palo alto, CA, USA) were cultured in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco) and 1% (v/v) penicillin/streptomycin (Gibco) and maintained at 37 °C with 5% CO₂. HUVECs were cultured in Endothelial Cell Growth Medium-2 (EGM-2) (Lonza, Basel, Switzerland) at 37 °C with 5% CO₂. The Expi293F cells (Gibco) were cultured in Expi293 expression medium (Gibco) in a humidified shaking incubator at 37 °C with 8% CO₂.

Lee J.H., Kim J.W., Yang H.R., Song S.W., Lee S.J., Jeon Y., Ju A., Lee N., Kim M.G., Kim M., Hwang K., Yoon J.H., Shim H, & Lee S. (2022). A Fully-Human Antibody Specifically Targeting a Membrane-Bound Fragment of CADM1 Potentiates the T Cell-Mediated Death of Human Small-Cell Lung Cancer Cells. International Journal of Molecular Sciences, 23(13), 6895.

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