Utilizing UV-visible JASCO V700 spectrophotometer (Model no. V-730, Serial no. B184461798 and JASCO Spectra Manager software), absorption spectra were recorded to check the binding affinity and binding constant at room temperature (25 °C). To perform this experiment two PerkinElmer quartz cell of path length of 1 cm were used for both reference and samples. The intensity vs. wavelength spectra for the absorbance measurement was recorded over the wavelength range 200–600 nm. For reference cell 10 mm phosphate buffer of pH 7.4 were taken. Concentration of the sample solutions were 13 μm.
Quartz cell
Manufactured by PerkinElmer
Sourced in Japan
The Quartz cell is a type of spectroscopic cell made of high-quality quartz glass. It is designed to hold liquid samples for analysis in various spectroscopic techniques, such as UV-Vis and infrared spectroscopy. The Quartz cell provides a transparent, inert, and durable container for the sample, allowing the passage of light through the sample for accurate measurements.
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Lab products found in correlation
3 protocols using quartz cell
1
UV-Vis Spectrophotometric Binding Assay
2
Circular Dichroism Analysis of Beta-Lactoglobulin
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Using Jasco spectropolarimeter (J-815) (Jasco, Tokyo, Japan) CD spectra of β-lg, heat treated β-lg and heat treated β-lg in presence of different coumarin compounds were recorded with the help of Jasco Spectra Manager Software. For this experiment in the far UV region (190–260 nm), each sample containing 13.6 μM β-lg was taken in a PerkinElmer quartz cell of path length of 0.2 cm and then data was recorded in the inner nitrogen atmosphere of the instrument at 190 nm to 260 nm. Scan speed was 100 nm min−1 and temperature was 30 °C. The results were expressed as mean residual ellipticity (MRE) in deg cm2 dmol−1 which is defined as: where θobs is the CD in millidegree, n is the number of amino acid residues in one subunit (162 for β-lg), l is the path length of the cell in centimeters and Cp is the molar fraction of proteins.
Spectra were recorded using Jasco Spectra Analysis tool. And secondary structural elements were calculated from CDNN 2.1 software.
Spectra were recorded using Jasco Spectra Analysis tool. And secondary structural elements were calculated from CDNN 2.1 software.
3
Selective Cu2+ Detection Using Probe 1
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Acetonitrile was used for preparing the stock solution of probe 1 (1 mM). The tested ions, namely Cu 2+ , Mn 2+ , Co 2+ , Ni 2+ , Zn 2+ , Cd 2+ , Ba 2+ , Ca 2+ , Ag + , Fe 3+ , K + , Na + , and Mg 2+ (10 mM), were prepared in Milli-Q water. To study the selectivity of probe 1 for Cu 2+ detection, the mixture solution of probe 1 and metal ions were prepared by adding the stock solution of probe 1 (20 μL) and the stock solution of each metal ion (10 μL) to the cell (1 cm) and then diluted by the addition of 2.5 mL acetonitrile. Absorption and fluorescence spectra were recorded after shaking the mixture for 5 min. For the Cu 2+ titration experiment, a solution of probe 1 (8 μM) in acetonitrile was prepared in a Perkin Elmer quartz cell (1 cm), to which was added the stock solution of Cu 2+ (0.1 equiv.). The mixture was shaken for 5 min before measuring UV-vis absorption and fluorescence spectra. This protocol was repeated until the addition of 8 equiv. in total. 47 The fluorescence quantum yields (ΦF) of probe 1 and probe 1 with Cu 2+ were measured by the standard method using Rhodamine 6G (Φstd = 0.95) in ethanol as a reference. 33
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