γ oryzanol

The Changlixiang brown rice was provided by Northeast Agricultural University, China. The ultrasonic equipment (BILON-1000CT, 20KHz) was provided by Shanghai bilang instrument manufacturing Co., LTD, China. The cellulase (700 U/g) was obtained from Furuidemu Biotechnology Co., LTD, China. The sodium bicarbonate and phosphate buffer were obtained from Beijing Solarbio Technology Co., LTD, China. The phytic acid, γ-oryzanol, Gamma-aminobutyric acid (GABA), phenolic acid and flavonoid standards were purchased from Shanghai Yuanye Biotechnology Co., LTD, China. The methanol, ethyl alcohol, acetonitrile and acetic acid used in the study were chromatographic grades and purchased from Shanghai Maclin Biochemical Technology Co., LTD, China.

Iron walnut kernels were purchased from a local factory in China, and stored at 4 °C after air drying. The anhydrous ethyl ether, 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH), total phenols, fatty acid methyl ester standard. The γ-oryzanol (purity ≥ 98.0 %), MGS, CW, BW, β-sitosterol (purity ≥ 95.0 %), cholesterol (purity ≥ 95.0 %) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The n-hexane, ethyl acetate, and other chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd (Hubei, China).