Cells were pelleted and washed with 1× PBS solution for preparation of microscope slides. A thin 2% (w/v) agarose pad was prepared on a glass slide and 1 μL of cell suspension was added on top and air dried for about 5 min before placing a glass cover slip (Ke et al., 2016 (link)). Imaging was performed with a DMi8 automated inverted microscope (Leica Microsystems, #11889113) equipped with a CCD camera (Leica Microsystems, #DFC300 G), and a TXR (Leica Microsystems, #11525310) and YFP (Leica Microsystems, #11525306) filter cube. The Z-stack of captured images was processed in a blind deconvolution to remove out of focus fluorescence with the LAS X software version 3.3.3.16958 (Leica Microsystems, #11640612) with 3D deconvolution package (Leica Microsystems, #11640865).
3d deconvolution package
Manufactured by Leica
The 3D deconvolution package is a software tool that enhances the clarity and resolution of 3D microscopic images. It utilizes advanced algorithms to remove the blurring effects caused by the optical system, enabling the visualization of finer details within the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 3d deconvolution package
1
Microscopy Slide Preparation and Imaging
2
Microscopic Imaging of Bacterial Cells
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Cells were pelleted and washed with 1× PBS solution for preparation of microscope slides.
A thin 2 % (w/v) agarose pad was prepared on a glass slide and 1 µL of cell suspension was added on top and air dried for about 5 min before placing a glass cover slip (Ke et al., 2016) (link).Imaging was performed with a DMi8 automated inverted microscope (Leica Microsystems, #11889113) equipped with a CCD camera (Leica Microsystems, #DFC300 G), and a TXR (Leica Microsystems, #11525310) and YFP (Leica Microsystems, #11525306) filter cube. The Z-stack of captured images was processed in a blind deconvolution to remove out of focus fluorescence with the LAS X software version 3.3.3.16958 (Leica Microsystems, #11640612) with 3D deconvolution package (Leica Microsystems, #11640865).
A thin 2 % (w/v) agarose pad was prepared on a glass slide and 1 µL of cell suspension was added on top and air dried for about 5 min before placing a glass cover slip (Ke et al., 2016) (link).Imaging was performed with a DMi8 automated inverted microscope (Leica Microsystems, #11889113) equipped with a CCD camera (Leica Microsystems, #DFC300 G), and a TXR (Leica Microsystems, #11525310) and YFP (Leica Microsystems, #11525306) filter cube. The Z-stack of captured images was processed in a blind deconvolution to remove out of focus fluorescence with the LAS X software version 3.3.3.16958 (Leica Microsystems, #11640612) with 3D deconvolution package (Leica Microsystems, #11640865).
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