Single cell suspensions (0.5 x 106 cells) were incubated for 15 min with Fc-receptor-blocking antibodies anti-CD16/anti-CD32 (BD Biosciences Pharmingen, San Diego, CA, USA), washed with PBS supplemented with 2% FCS and then stained for 30 min with the indicated conjugated antibodies. Cells were washed twice and fixed with 0.37% formaldehyde in PBS. FITC-conjugated anti-CD19, PE-conjugated anti-CD3, APC-conjugated anti-CD4, BV410-conjugated anti-CD8, APC-conjugated anti-CD11b, BV711-conjugated anti-CD11c, FITC-conjugated anti-Gr-1 and PE-conjugated anti-F4/80, were purchased from eBioscience (San Diego, CA, USA). Cells were analysed in a FACS Fortessa flow cytometer and data were processed with the FlowJo software (Becton Dickinson Labware, Franklin Lakes, NJ, USA).
Fitc conjugated anti cd19
Manufactured by Thermo Fisher Scientific
Sourced in United States
FITC-conjugated anti-CD19 is a monoclonal antibody that specifically binds to the CD19 antigen expressed on the surface of B cells. The antibody is conjugated with the fluorescent dye Fluorescein isothiocyanate (FITC), which allows for the detection and identification of B cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Fitc conjugated anti cd4, by Thermo Fisher Scientific (3 mentions)
Pe conjugated anti icos, by Thermo Fisher Scientific (2 mentions)
Pe conjugated anti icosl, by Thermo Fisher Scientific (2 mentions)
Pe conjugated anti cd3, by Thermo Fisher Scientific (1 mentions)
Apc conjugated anti cd11b, by Thermo Fisher Scientific (1 mentions)
Facs fortessa flow cytometer, by BD (1 mentions)
Apc conjugated anti cd4, by Thermo Fisher Scientific (1 mentions)
Fitc conjugated anti gr 1, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti f4 80, by Thermo Fisher Scientific (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Pe conjugated anti cd8, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti nk1, by Thermo Fisher Scientific (1 mentions)
Apc conjugated anti cd11c, by Thermo Fisher Scientific (1 mentions)
Fitc conjugated anti cd11b, by Thermo Fisher Scientific (1 mentions)
Apc conjugated anti cd3, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti ly6g, by Thermo Fisher Scientific (1 mentions)
Facs flow cytometer, by BD (1 mentions)
Fix perm reagents, by Thermo Fisher Scientific (1 mentions)
Allophycocyanin apc conjugated anti cd4, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti pd l1, by Thermo Fisher Scientific (1 mentions)
4 protocols using fitc conjugated anti cd19
1
Multiparametric Flow Cytometry Immunophenotyping
2
Flow Cytometry Analysis of Splenocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, 1×106 splenocytes were incubated in 100 μl of PBS. The cells were then stained with a mixture of PE- and FITC-conjugated mAbs for 30 min, washed twice, then fixed with 4% paraformaldehyde in PBS. The analysis was performed on a FACS Calibur Flow Cytometer (BD Biosciences). All procedures were performed on ice until the time of analysis. The mAbs for flow cytometry were purchased from eBioscience (San Diego, USA) and included FITC-conjugated anti-CD4, FITC-conjugated anti-CD19, PE-conjugated anti-ICOS, PE-conjugated anti-ICOSL, PE-conjugated anti-RORγtand PE-conjugated anti-IL-17R.
3
Flow Cytometry Analysis of Immune Cell Subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LP cells from colon or splenic immune cells were sorted using a FACS flow cytometer (BD Biosciences, San Jose, CA) and analyzed using FlowJo_V10 (Treestar, Ashlan, OR). The gating strategy has been shown in supplementary Figure 8. For macrophage and neutrophil analyses, cells were incubated with the following antibodies: FITC-conjugated anti-CD11b (11-0112-82, eBioscience, San Diego, CA), APC-conjugated anti-CD11c (17-0114-81, eBioscience), PE-cyanine7-conjugated anti-F4/80 (25-4801-82, eBioscience), PE-conjugated anti-CD206 (12-2061-80, eBioscience), and PE-conjugated anti-Ly6G (12-5931-81, eBioscience); for T cell analyses, cells were incubated with the following antibodies: APC-conjugated anti-CD3 (17-0032-82, eBioscience), FITC-conjugated anti-CD4 (11-0042-81, eBioscience), and PE-conjugated anti-CD8 (12-0081-81, eBioscience); for B cell and NK cell analyses, cells were incubated with the following antibodies: FITC-conjugated anti-CD19 (11-0193-81, eBioscience), and PE-conjugated anti-NK1.1 (12-5941-81, eBioscience).
4
Characterizing Hepatic and Splenic Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lymphomononuclear cells from the livers, spleens and peripheral blood were incubated with fluorochrome-labeled antibodies at 5×105 / tube for 30 min at 4°C, and characterized using a FACS Canto II cytometer (BD Biosciences). For the intracellular cytokine staining, CaltagTM, Fix&Perm® reagents (Invitrogen, Carlsbad, CA, USA) were used following the manufacturer's instructions. The following antibodies were used: fluorescein isothiacyanate (FITC)-conjugated anti-CD4, FITC-conjugated anti-CXCR5, FITC-conjugated anti-CD19, Phycoerythrin (PE)-conjugated anti-PD-1, PE-conjugated anti-ICOS, PE-conjugated anti-PDL-1, PE-conjugated anti-ICOSL, PE-conjugated anti-IL-21R, allophycocyanin (APC)-conjugated anti-CD4 (eBioscience, USA), PE-conjugated anti-IL-21 (BD Biosciences, USA) and FITC-, APC- or PE-conjugated isotype antibodies (eBioscience, USA).
Lymphomononuclear cells from the livers and spleens were incubated with HBc-derived peptides HBc1-20 (1 mg / ml)at room temperature for 10 min. HBc-derived peptides HBc1-20 was purchased from Sangon Biotech (Sangon, Shanghai, China). The cells were then washed twice with PBS containing 1% BSA (BD Biosciences), and incubated with anti-CD4, anti-CXCR5 for 30 min at 4°C, and characterized using a FACS Canto II cytometer.
Lymphomononuclear cells from the livers and spleens were incubated with HBc-derived peptides HBc1-20 (1 mg / ml)at room temperature for 10 min. HBc-derived peptides HBc1-20 was purchased from Sangon Biotech (Sangon, Shanghai, China). The cells were then washed twice with PBS containing 1% BSA (BD Biosciences), and incubated with anti-CD4, anti-CXCR5 for 30 min at 4°C, and characterized using a FACS Canto II cytometer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!