All animal husbandry and procedures were performed in compliance with institutional animal care and use committee guidelines. Crhr2-ires-Cre and Gabra1-ires-Cre mice were constructed as described below; Gpr65-ires-Cre, Glp1r-ires-Cre, Npy2r-ires-Cre, P2ry1-ires-Cre and P2×2/P2×3−/− mice were described before (Chang et al., 2015 (link); Finger et al., 2005 ; Williams et al., 2016 (link)); Vglut2-ires-Cre and loxP-L10-GFP mice were gifts from Bradford Lowell (Beth Israel Deaconess Medical Center); and wild type C57BL/6J (000664), Calb1-ires-Cre (028532), Chat-ires-Cre (006410), Chat-GFP (007902), Krt8-CreER (017947), Npy1r-Cre (030544), Piezo2-EGfp-ires-Cre (027719), Plcβ2−/− (018064), loxP-tdTomato (007908), loxP-ChR2 (012569), and loxP-DTR (007900) mice were purchased (Jackson Laboratory). The constitutive GCaMP3 allele (Rosa26-GCaMP3) was generated by breeding loxP-GCaMP3 (014538) with E2a-Cre mice (Jackson, 003314), and then crossing out the E2a-Cre allele. Single-cell sequencing was performed on male C57BL/6J mice; both male and female mice between 8–24 weeks old were used for all other studies, and no differences based on sex were observed.
Loxp chr2
Manufactured by Jackson ImmunoResearch
LoxP-ChR2 is a genetic construct containing the Cre recombinase recognition sequence (LoxP) and the light-sensitive channelrhodopsin-2 (ChR2) gene. This product allows for cell-specific expression and optical control of neuronal activity.
Automatically generated - may contain errors
Lab products found in correlation
Loxp dtr, by Jackson ImmunoResearch (3 mentions)
Piezo2 egfp ires cre, by Jackson ImmunoResearch (3 mentions)
Mc4r 2a cre, by Jackson ImmunoResearch (2 mentions)
C57bl 6j, by Jackson ImmunoResearch (2 mentions)
Chat ires cre, by Jackson ImmunoResearch (1 mentions)
E2a cre mice, by Jackson ImmunoResearch (1 mentions)
Chat gfp, by Jackson ImmunoResearch (1 mentions)
Loxptdtomato, by Jackson ImmunoResearch (1 mentions)
Krt8 creer, by Jackson ImmunoResearch (1 mentions)
Pv cre, by Jackson ImmunoResearch (1 mentions)
Sst cre, by Jackson ImmunoResearch (1 mentions)
4 protocols using loxp chr2
1
Cre-Dependent Mouse Lines for Neuroscience Research
2
Genetically Modified Mouse Models
3
Genetic Mouse Models for Optogenetic Neuroscience
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All procedures involving animals were approved by and performed in accordance with the guidelines of the Kantonales Veterinäramt Basel-Stadt, Switzerland. Mice were maintained on 12-hour light/dark cycle with water and food available ad libitum. Male and female mice were used, unless indicated otherwise. Age of the animals is indicated in individual experiments, in general mice were used at P25-30 for biochemical and immunohistochemical analysis and at 6-8 weeks for electrophysiological recordings.
The following mice strains were used in this study: LoxP-ChR2 (JAX: 024109; Madisen et al., 2012 (link)), PV-Cre (JAX: 017320; Hippenmeyer et al., 2005 (link)) and SST-Cre (JAX: 013044; Taniguchi et al., 2011 (link)) mice were obtained from Jackson Laboratories, RjOrl:Swiss mice (CD-1) were from Janvier Labs, for generation of Nrxn3ΔEx24 knock-out and Nrxn3 AS5HA knock-in mice seeSTAR Methods details. All mouse lines were maintained on a C57BL6/J strain background.
In general, het/het breeding schemes were used for Nrxn3ΔEx24 knock-out and Nrxn3 AS5HA knock-in mice. For Cre mediated ChR2 expression, homozygous PV- or SST-Cre animals (heterozygous for Nrxn3ΔEx24) were mated with homozygous LoxP-ChR2 animals (heterozygous for Nrxn3ΔEx24), and experiments were performed with 6-8 week old mice heterozygous for PV- and SST-Cre or LoxP-ChR2.
The following mice strains were used in this study: LoxP-ChR2 (JAX: 024109; Madisen et al., 2012 (link)), PV-Cre (JAX: 017320; Hippenmeyer et al., 2005 (link)) and SST-Cre (JAX: 013044; Taniguchi et al., 2011 (link)) mice were obtained from Jackson Laboratories, RjOrl:Swiss mice (CD-1) were from Janvier Labs, for generation of Nrxn3ΔEx24 knock-out and Nrxn3 AS5HA knock-in mice see
In general, het/het breeding schemes were used for Nrxn3ΔEx24 knock-out and Nrxn3 AS5HA knock-in mice. For Cre mediated ChR2 expression, homozygous PV- or SST-Cre animals (heterozygous for Nrxn3ΔEx24) were mated with homozygous LoxP-ChR2 animals (heterozygous for Nrxn3ΔEx24), and experiments were performed with 6-8 week old mice heterozygous for PV- and SST-Cre or LoxP-ChR2.
4
Genetically Modified Mouse Models
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All animal husbandry and procedures were performed in compliance with institutional animal care and committee guidelines. Gpr65-ires-Cre, Glp1r-ires-Cre, and Phox2b-Cre; loxP-Piezo1; loxP-Piezo2 mice were described before (Chang et al., 2015 (link); Williams et al., 2016 (link); Zeng et al., 2018 (link)); Vglut2-ires-Cre mice were a generous gift from Bradford Lowell (Beth Israel Deaconess Medical Center); and wild-type C57BL/6J (000664), Piezo2-EGfp-ires-Cre (027719), Mc4r-2a-Cre (030759), LoxP-ChR2 (012569), and LoxP-DTR (007900) mice were purchased (Jackson). Male and female mice between 8–16 weeks old were used for all studies, and no differences based on sex were observed.
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