Cotinine elisa co096d

Manufactured by Calbiotech

Sourced in United States

The Cotinine ELISA CO096D is a quantitative enzyme-linked immunosorbent assay (ELISA) kit designed to detect and measure the concentration of cotinine, a metabolite of nicotine, in various biological samples. The kit utilizes a competitive ELISA principle and provides a reliable method for the assessment of nicotine exposure.

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Cotinine ELISA (2 citations) CO096D (2 citations) Cotinine (2 citations) ELISAs (2 citations)

5 protocols using cotinine elisa co096d

Cotinine Measurement in Pregnant Women

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Cotinine was measured in a subsample of women with stored urine samples collected at ~27 weeks gestation. Cotinine was measured via. solid phase competitive ELISA, with a sensitivity of 1 ng/mL (Calbiotech Cotinine ELISA CO096D, Calbiotech, El Cajon, California). The limit of detection (LOD) was 0.05 ng/mL. We categorized women as either nonsmoker (cotinine < 31.5 ng/mL; the established cutpoint for active smoking^{18 (link)}) or active smoker (≥31.5 ng/mL).

Moore B.F., Starling A.P., Martenies S.E., Magzamen S, & Dabelea D. (2021). Joint effects of ambient air pollution and maternal smoking on neonatal adiposity and childhood BMI trajectories in the Healthy Start study. Environmental Epidemiology, 5(3), e142.

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Prenatal Cotinine Exposure Assessment

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Cotinine was measured in a subsample of women with stored urine samples collected at ~ 27 weeks gestation. Cotinine was measured via solid phase competitive ELISA, with a sensitivity of 1 ng/mL (Calbiotech Cotinine ELISA CO096D). The limit of detection (LOD) was 0.05 ng/mL. The cotinine categories were defined as follows: no exposure (< LOD), exposure to secondhand smoke (≥ LOD to 550 ng/mL; the established cutpoint for active smoking [17 (link)]), and active smoking (≥ 550 ng/mL).
Self-report of active smoking and exposure to second hand smoke prenatal smoking was ascertained through questionnaires completed at ~ 27 weeks gestation. Self-report of exposure was defined as follows: no exposure (if they reported not currently smoking and no secondhand smoke exposure), exposure to secondhand smoke (if they reported not currently smoking and > 1 h/week exposure to secondhand smoke), and active smoking (if they reported current smoking).

Moore B.F., Starling A.P., Magzamen S., Harrod C.S., Allshouse W.B., Adgate J.L., Ringham B.M., Glueck D.H, & Dabelea D. (2018). Fetal exposure to maternal active and secondhand smoking with offspring early-life growth in the Healthy Start study. International journal of obesity (2005), 43(4), 652-662.

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Prenatal Tobacco Exposure Assessment

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Prenatal exposure to tobacco was assessed via urinary cotinine (major metabolite of nicotine) at ~ 27 weeks gestation. Cotinine was previously analyzed in stored urine samples via solid phase competitive ELISA with a sensitivity of 1 ng/ mL (Calbiotech Cotinine ELISA CO096D, Calbiotech, El Cajon, California). Consistent with a previous Healthy Start analysis [5] , cotinine concentrations were dichotomized as no exposure (< 0.05 ng/mL; the limit of detection [LOD]) and prenatal exposure to exposure (> LOD).

Hoyt A.T., Wilkinson A.V., Langlois P.H., Galeener C.A., Ranjit N., Dabelea D.M, & Moore B.F. (2023). Prenatal Exposure to Tobacco and Childhood Cognition and Behavior: Effect Modification by Maternal Folate Intake and Breastfeeding Duration. Child psychiatry and human development.

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Maternal Tobacco Exposure Biomarker Assessment

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Urinary cotinine (a metabolite of nicotine and marker of tobacco exposure [15 (link)]) was measured in a subsample of study participants at ~ 27 weeks gestation. Cotinine was analyzed in stored urine samples via solid phase competitive ELISA—with a sensitivity of 1 ng/mL (Calbiotech Cotinine ELISA CO096D, Calbiotech, El Cajon, California). Following a previous Healthy Start analysis examining similar exposures, [16 (link)] cotinine concentrations were categorized as follows: no exposure (< limit of detection [LOD]); ~ 0.05 ng/mL), maternal exposure to secondhand smoke (cotinine ≥ LOD to 550 ng/mL), and active maternal smoking (≥ 550 ng/mL; an established cut-point for active smoking [17 (link)]). Very few women were active smokers (~ 6%). Therefore, cotinine was dichotomized as no exposure (< LOD; 74%) and any exposure to tobacco (≥ LOD; 26%) to maximize power in our interaction analyses.

Hoyt A.T., Wilkinson A.V., Langlois P.H., Galeener C.E., Ranjit N., Sauder K.A., Dabelea D.M, & Moore B.F. (2022). Prenatal exposure to tobacco and adverse birth outcomes: effect modification by folate intake during pregnancy. Maternal Health, Neonatology and Perinatology, 8, 6.

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Urine Cotinine Levels and Smoking

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Urine cotinine levels were determined using the Calbiotech Cotinine ELISA CO096D (Calbiotech, El Cajon, California, USA) and the limit of detection was 5 ng/mL. A cotinine-verified non-smoker was defined as having urinary cotinine ≤50 ng/mL. This threshold value was used as the cut-off according to the Society for Research on Nicotine and Tobacco and is consistent with being a current smoker.22 (link)

Butler-Dawson J., Barnoya J., Brindley S., Krisher L., Fan W., Asensio C, & Newman L.S. (2021). Cross-sectional study examining the accuracy of self-reported smoking status as compared to urinary cotinine levels among workers at risk for chronic kidney disease of unknown origin in Guatemala. BMJ Open, 11(10), e050374.

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